Dong Y L, Liu W, Gao Y M, Wu R D, Zhang Y H, Wang H F, Wei B
Department of Histology, Shandong University School of Medicine, Jinan, China.
Transplant Proc. 2008 Dec;40(10):3646-52. doi: 10.1016/j.transproceed.2008.06.107.
Neural stem cell (NSC) transplantation is a promising tool for restoring the nervous system in a variety of neurodegenerative disorders. The aim of this study was to determine the potential of NSC transplantation as a therapeutic strategy for neuronal replacement of the enteric nervous system of the rectum in an aganglionic rat.
Rat central nervous system-derived NSCs (CNS-NSCs) obtained from the cortex of the fetal brain (E16) were transplanted into the benzalkonium chloride (BAC)-induced rat aganglionic rectum. Survival and differentiation of the implanted cells were assessed at 8 weeks posttransplantation using immunostaining and Western blotting. The rectoanal inhibitory reflex (RAIR) was also be measured.
Eight weeks following transplantation, grafted CNS-NSCs differentiated into neurons and glial cells in the aganglionic rectum. The protein expression of neuronal nitric oxide synthase (nNOS) and choline acetyltransferase (ChAT) were significantly increased and the RAIR restored after cell implantation.
CNS-NSC transplantation may provide a viable therapeutic option for disorders of the enteric nervous system.
神经干细胞（NSC）移植是恢复多种神经退行性疾病中神经系统功能的一种有前景的手段。本研究的目的是确定NSC移植作为一种治疗策略，用于无神经节大鼠直肠肠神经系统神经元替代的潜力。
从胎脑（E16）皮质获得的大鼠中枢神经系统来源的神经干细胞（CNS-NSCs）被移植到苯扎氯铵（BAC）诱导的大鼠无神经节直肠中。在移植后8周，使用免疫染色和蛋白质印迹法评估植入细胞的存活和分化情况。还测量了直肠肛门抑制反射（RAIR）。
移植后8周，移植的CNS-NSCs在无神经节直肠中分化为神经元和胶质细胞。细胞植入后，神经元型一氧化氮合酶（nNOS）和胆碱乙酰转移酶（ChAT）的蛋白表达显著增加，RAIR恢复。
CNS-NSC移植可能为肠神经系统疾病提供一种可行的治疗选择。
