Liu Xiumei, Wang Yuwei, Li Dong, Ju Xiuli
Department of Pediatrics, Qilu Hospital of Shandong University, Jinan, Shandong 250012, China.
Behav Brain Res. 2008 Jan 10;186(1):84-90. doi: 10.1016/j.bbr.2007.07.040. Epub 2007 Aug 6.
Tourette syndrome (TS) is a heterogenous neuropsychiatric disorder. In most cases, tics are self-limited or can be treated by behavioral or pharmacological therapy. However, for some individuals, tics can cause lifelong impairment and life-threatening symptoms, which are intractable to traditional treatment. Neural stem cell (NSC) is a potential tool to treat certain neurological diseases. In this study, we proposed to use neural stem cell transplantation as a novel therapy to treat TS and discussed its efficacy. Wistar rats were microinfused with TS sera into the striatum followed by the transplantation of NSCs or vehicle at the infusion site. The sera of the TS patients were identified to have enriched antineural antibodies. Prior to grafting, rat embryonic NSCs were co-cultured with 5-bromodeoxyuridine (Brdu) for 24 h. Stereotypic behaviors were counted at 1, 7, 14 and 21 days after transplantation of NSCs. Morphological analyses revealed that NSCs survived and differentiated into neurons and astrocytes in the striatum 3 weeks after grafting. To sum it up, rat embryonic neural stem cell grafts survived and differentiated in the striatum of TS rat may help relieve stereotypic behaviors of the host. Our results suggest that transplantation of NSCs intrastriatum may have therapeutic potential for TS.
抽动秽语综合征（TS）是一种异质性神经精神障碍。在大多数情况下，抽动症状是自限性的，或者可以通过行为或药物治疗。然而，对于一些个体来说，抽动症状可能会导致终身功能障碍和危及生命的症状，这些症状对传统治疗方法难以奏效。神经干细胞（NSC）是治疗某些神经系统疾病的一种潜在工具。在本研究中，我们提出使用神经干细胞移植作为一种治疗TS的新疗法，并讨论了其疗效。将TS患者血清微量注入Wistar大鼠纹状体，然后在注射部位移植神经干细胞或赋形剂。已确定TS患者的血清中富含抗神经抗体。在移植前，将大鼠胚胎神经干细胞与5-溴脱氧尿苷（Brdu）共培养24小时。在移植神经干细胞后1、7、14和21天对刻板行为进行计数。形态学分析显示，移植3周后，神经干细胞在纹状体中存活并分化为神经元和星形胶质细胞。综上所述，大鼠胚胎神经干细胞移植在TS大鼠纹状体中存活并分化，可能有助于减轻宿主的刻板行为。我们的结果表明，纹状体内移植神经干细胞可能对TS具有治疗潜力。
