Daley Adam B, Oleschuk Richard D
Department of Chemistry, Queen's University, Kingston, ON, Canada.
J Chromatogr A. 2009 Jan 30;1216(5):772-80. doi: 10.1016/j.chroma.2008.11.078. Epub 2008 Dec 3.
With applications that take advantage of highly selective fluorine-fluorine interactions appearing with greater frequency in the literature, the development of porous polymer monoliths from fluorous components is reported here for the purpose of chromatography of tagged analytes. With potential uses in fields as diverse as separation science and proteomics, facile fabrication of materials with fluorous specificity that can be applied in a high-throughput manner is greatly desirable. To this end, we have developed porous polymer capillary columns with varied fluorous content using a simple UV-initiated radical polymerization process and characterized them using flow-induced backpressure and scanning electron microscopy (SEM). With structural similarities assured (visually, and by backpressure variations of less than 42%), the monoliths were tested as chromatographic columns for the separation of a series of fluorous-tagged analytes under gradient conditions. It was found that columns made with fluorinated components exhibited greater selectivity for fluorous analytes than did equivalent, non-fluorinated monoliths, retaining analytes with either one or two fluorous tags for approximately 6% and 13% longer, respectively. This supports the idea of differences existing between fluorous and reverse-phase separation mechanisms, and encourages a broader range of potential applications for fluorous monoliths of this type.
随着文献中利用高选择性氟-氟相互作用的应用越来越频繁地出现，本文报道了由含氟成分制备多孔聚合物整体柱，用于标记分析物的色谱分析。鉴于其在分离科学和蛋白质组学等不同领域的潜在用途，非常需要能够以高通量方式应用的具有氟特异性的材料的简便制备方法。为此，我们使用简单的紫外光引发自由基聚合工艺开发了具有不同氟含量的多孔聚合物毛细管柱，并通过流动诱导背压和扫描电子显微镜（SEM）对其进行了表征。在确保结构相似性（通过视觉观察以及背压变化小于42%）的情况下，将这些整体柱作为色谱柱进行测试，以在梯度条件下分离一系列含氟标记的分析物。结果发现，与等效的非氟化整体柱相比，含氟成分制备的柱对含氟分析物具有更高的选择性，保留带有一个或两个氟标记的分析物的时间分别长约6%和13%。这支持了氟相和反相分离机制之间存在差异的观点，并鼓励对这类含氟整体柱进行更广泛的潜在应用探索。
