Willmann M, Müllauer L, Guija de Arespacochaga A, Reifinger M, Mosberger I, Thalhammer J G
Department for Companion Animals and Horses, Clinic for Internal Medicine and Infectious Diseases, University of Veterinary Medicine Vienna, Vienna, Austria.
Vet Immunol Immunopathol. 2009 Apr 15;128(4):359-65. doi: 10.1016/j.vetimm.2008.11.019. Epub 2008 Nov 13.
The Pax5 gene encodes the B-cell specific activator protein (BSAP), a member of the highly conserved paired box (PAX)-domain family of transcription factors and a key regulator in the development and differentiation of B-cells. Pax5 serves as a valuable B-cell marker in the classification of human lymphoma patients as it is restricted to lymphomas of B-cell lineage. In dogs, detection of Pax5 protein in lymphoma tissue has not been reported. Therefore, we have investigated the expression and detection of BSAP using a monoclonal anti-Pax5 antibody (anti-BSAP, clone 24) in canine lymphoma tissue samples to evaluate its diagnostic relevance as a B-cell marker. A series of 25 lymph nodes from 23 canine non-Hodgkin lymphoma patients, a reactive canine lymph node, and a normal non-reactive canine lymph node, were evaluated. All B-cell non-Hodgkin lymphomas (15) were found to express Pax5 protein. In addition, there was a strong correlation between Pax5 and CD79a expression. Three CD3 positive and five CD3 and CD79a positive lymphomas were immunophenotypically negative for anti-Pax5, indicating a T-cell lineage. In conclusion, anti-Pax5 antibody may offer an excellent B-cell marker in canine lymphomas.
Pax5基因编码B细胞特异性激活蛋白（BSAP），它是转录因子中高度保守的配对盒（PAX）结构域家族的成员，也是B细胞发育和分化的关键调节因子。由于Pax5仅限于B细胞系淋巴瘤，因此它是人类淋巴瘤患者分类中有价值的B细胞标志物。在犬类中，尚未见淋巴瘤组织中Pax5蛋白检测的报道。因此，我们使用单克隆抗Pax5抗体（抗BSAP，克隆24）研究了犬淋巴瘤组织样本中BSAP的表达和检测，以评估其作为B细胞标志物的诊断相关性。我们评估了来自23例犬非霍奇金淋巴瘤患者的25个淋巴结、一个反应性犬淋巴结和一个正常无反应性犬淋巴结。所有B细胞非霍奇金淋巴瘤（15例）均表达Pax5蛋白。此外，Pax5与CD79a表达之间存在强相关性。3例CD3阳性和5例CD3与CD79a阳性淋巴瘤对抗Pax5免疫表型呈阴性，表明为T细胞系。总之，抗Pax5抗体可能是犬淋巴瘤中一种出色的B细胞标志物。
