一种用于同时检测犬猫细胞学样本中CD20和CD3的快速多重免疫荧光方法。
A rapid multiple immunofluorescence method for the simultaneous detection of CD20 and CD3 in canine and feline cytological samples.
作者信息
Matsumoto Fumi, Shima-Sawa Mariko, Furusawa Yu, Yamato Osamu, Yabuki Akira
机构信息
Laboratory of Veterinary Clinical Pathology, Joint Faculty of Veterinary Medicine, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan.
Veterinary Teaching Hospital, Joint Faculty of Veterinary Medicine, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan.
出版信息
J Vet Med Sci. 2021 May 17;83(5):832-836. doi: 10.1292/jvms.20-0597. Epub 2021 Apr 5.
CD20 and CD3 are considered reliable markers for B and T cells, respectively. This study aimed to develop a rapid multiple immunofluorescence (RMIF) method for the detection of CD20 and CD3 on a single cytology slide. Air-dried smears were prepared using samples collected from dogs (n=26) and cats (n=6). Immunosignal detection using the newly developed method required 60 min. Clear immunosignals for CD20 and CD3 were detected in 24 of 26 samples in dogs and in all 6 cats. As the RMIF (CD20/CD3) method can detect markers of both B and T cells simultaneously on a single cytology smear, it would be an efficient tool for the immunophenotyping of canine and feline lymphoma samples.
CD20和CD3分别被认为是B细胞和T细胞的可靠标志物。本研究旨在开发一种快速多重免疫荧光（RMIF）方法，用于在单一细胞学载玻片上检测CD20和CD3。使用从犬（n=26）和猫（n=6）采集的样本制备空气干燥涂片。使用新开发的方法进行免疫信号检测需要60分钟。在26只犬的24个样本和所有6只猫中均检测到清晰的CD20和CD3免疫信号。由于RMIF（CD20/CD3）方法可以在单一细胞学涂片上同时检测B细胞和T细胞的标志物，因此它将是犬猫淋巴瘤样本免疫表型分析的有效工具。
Zotero 插件