Gao Dong, Zheng Zhong, Han Mingfei, Tang Xiangdong, Sun Xueli
Mental Health Center, West China Hospital, Sichuan University, Chengdu 610041, PR China.
Int J Psychophysiol. 2009 May;72(2):160-5. doi: 10.1016/j.ijpsycho.2008.12.002. Epub 2008 Dec 7.
The aim of this study was to explore whether there were abnormalities of CNV-like and P(300)-like potentials in stressed rats following repeatedly forced swim stress. Forty male rats were randomly divided into 4 groups: the control groups (Control-1 and Control-2) and the stressed groups (Stress-1 and Stress-2). Rats in stressed groups were administered to repeatedly forced swim 7 or 14 days respectively. Body weight gain, saccharin preference test and open field test were performed. After being anesthetized with urethane, P(300)-like potentials were evoked by the oddball auditory stimulation and CNV-like potentials were elicited by condition-test stimulus. Results of behavioral tests displayed less body weights and less saccharine solution intake in stressed rats and significant effects of stress on the number of crossing squares, the duration of rearing and the number of grooming in open field test. Prolonged P3 latencies and decreased P3 amplitudes of P(300)-like potentials were found in the stressed rats. CNV amplitudes were lower in the stressed rats than those in control. Moreover, there were significant correlations between parameters of ERPs (including P3 latency, amplitude and CNV amplitude) and a serial of behavioral traits. This study provides an important evidence of changes of CNV-like and P(300)-like potentials in depressed rats following repeatedly forced swim stress. Based on this study, ERPs should be taken into consideration and applied as the useful tools in the research work of depressed animal models.
本研究旨在探讨反复强迫游泳应激后应激大鼠是否存在类CNV电位和类P(300)电位异常。40只雄性大鼠随机分为4组：对照组（Control-1和Control-2）和应激组（Stress-1和Stress-2）。应激组大鼠分别进行7天或14天的反复强迫游泳。进行体重增加、糖精偏好试验和旷场试验。用乌拉坦麻醉后，用奇偶数听觉刺激诱发类P(300)电位，用条件-测试刺激诱发类CNV电位。行为测试结果显示，应激大鼠体重减轻，糖精溶液摄入量减少，应激对旷场试验中穿越方格的数量、直立持续时间和理毛次数有显著影响。在应激大鼠中发现类P(300)电位的P3潜伏期延长，P3波幅降低。应激大鼠的CNV波幅低于对照组。此外，ERP参数（包括P3潜伏期、波幅和CNV波幅）与一系列行为特征之间存在显著相关性。本研究为反复强迫游泳应激后抑郁大鼠类CNV电位和类P(300)电位的变化提供了重要证据。基于本研究，ERP应被考虑并作为抑郁动物模型研究工作中的有用工具加以应用。
