Sonoda F, Oishi K, Tanaka H, Hideaki M, Kobayashi S, Nagatake T, Matsumoto K
Department of Internal Medicine, Nagasaki University, Japan.
Nihon Kyobu Shikkan Gakkai Zasshi. 1991 Jun;29(6):703-9.
To elucidate the clinical significance of serum-sensitivity of respiratory pathogenic Pseudomonas aeruginosa (P. aeruginosa) strains, we examined serum-sensitivity of P. aeruginosa isolated from 16 patients with lower respiratory tract infections and clinical backgrounds of these patients. We also evaluated the virulence of four serum-resistant and four serum-sensitive P. aeruginosa strains in murine pneumonia model induced by intratracheal challenge, and the silver-stained profiles of purified lipopolysaccharide (LPS) from these strains. Serum-sensitive strains were isolated only from patients with chronic bronchitis, bronchiectasis, and diffuse panbronchiolitis colonized with P. aeruginosa, and rarely caused pneumonias, while serum-resistant strains caused pneumonias in some cases. Intratracheal challenge of mice with 5 x 10(7) cfu per mouse of a serum resistant strain caused fatal hemorrhagic pneumonia with bacteremia. In contrast, the same dose of a serum-sensitive strain provided non-fatal pneumonia without bacteremia. LD50 of serum-sensitive strains in a murine model of P. aeruginosa pneumonia were at least 2-10 times higher than those of serum-resistant strain. The LPS profiles of two serum-resistant strains and one serum-sensitive strain showed ladder-like patterns. The similar analysis demonstrated that one serum-sensitive strain was lack of ladder-like patterns. These data support that serum-sensitive P. aeruginosa strains are less virulent than serum-resistant P. aeruginosa strains in the lower respiratory tract, and serum sensitivity of P. aeruginosa strains is determined by the structure of the O-side chain of LPS; either lack of the O-side chain or the presence of sparse O-side chain.
为阐明呼吸道致病性铜绿假单胞菌菌株血清敏感性的临床意义，我们检测了从16例下呼吸道感染患者分离出的铜绿假单胞菌的血清敏感性以及这些患者的临床背景。我们还在气管内接种诱导的小鼠肺炎模型中评估了4株血清耐药和4株血清敏感铜绿假单胞菌菌株的毒力，以及这些菌株纯化脂多糖（LPS）的银染图谱。血清敏感菌株仅从慢性支气管炎、支气管扩张症和弥漫性泛细支气管炎合并铜绿假单胞菌定植的患者中分离得到，很少引起肺炎，而血清耐药菌株在某些情况下会引起肺炎。用每只小鼠5×10⁷ cfu的血清耐药菌株对小鼠进行气管内接种，会导致致命的出血性肺炎并伴有菌血症。相比之下，相同剂量的血清敏感菌株引起的是非致命性肺炎且无菌血症。在铜绿假单胞菌肺炎小鼠模型中，血清敏感菌株的半数致死剂量（LD50）比血清耐药菌株至少高2至10倍。两株血清耐药菌株和一株血清敏感菌株的LPS图谱呈梯状模式。类似分析表明，一株血清敏感菌株缺乏梯状模式。这些数据支持在呼吸道中血清敏感铜绿假单胞菌菌株的毒力低于血清耐药铜绿假单胞菌菌株，并且铜绿假单胞菌菌株的血清敏感性由LPS的O侧链结构决定；即缺乏O侧链或存在稀疏的O侧链。
