两种果蝇发育过程中保幼激素代谢酶的比较分析
A comparative analysis of juvenile hormone metabolyzing enzymes in two species of Drosophila during development.
作者信息
Khlebodarova T M, Gruntenko N E, Grenback L G, Sukhanova M Z, Mazurov M M, Rauschenbach I Y, Tomas B A, Hammock B D
机构信息
Institute of Cytology and Genetics, Siberian Department, Russian Academy of Sciences, Novosibirsk, Russia.
出版信息
Insect Biochem Mol Biol. 1996 Sep-Oct;26(8-9):829-35. doi: 10.1016/s0965-1748(96)00043-4.
The course of changes in the activities of enzymes degrading juvenile hormone (JH), epoxyde hydrolase (JHEH) and JH-esterase (JHE) was studied in two lines of Drosophila virilis (101 and 147) and in two lines of D. melanogaster (Canton-S and 921283). It was established for D. virilis that changes in the JH titre during pupal-adult development is determined by the activity level of JHE rather then JHEH, while in D. melanogaster developmental changes in JH titre are related to changes in the activity level of both JHE and JHEH. In adults of D. virilis, the high level of JH-hydrolysing activity is determined by JHE and in those of D. melanogaster by JHEH. Differences in the course of changes in the JHE activity level between adults of lines 101 and 147 of D. virilis were found, and also in the JHEH activity level between adults of lines Canton S and 921283 of D. melanogaster. It was shown that attainment of a definite JHE activity level in females of lines 101 and 147 agrees well with the onset of oviposition of fertilized eggs. The possible role of JHE in reproduction of D. virilis is discussed.
研究了两条粗壮果蝇品系（101和147）以及两条黑腹果蝇品系（Canton-S和921283）中降解保幼激素（JH）的酶——环氧水解酶（JHEH）和JH酯酶（JHE）的活性变化过程。已确定，对于粗壮果蝇而言，蛹期到成虫发育过程中JH滴度的变化是由JHE而非JHEH的活性水平决定的，而对于黑腹果蝇，JH滴度的发育变化与JHE和JHEH的活性水平变化均有关。在粗壮果蝇成虫中，JH水解活性的高水平由JHE决定，而在黑腹果蝇成虫中则由JHEH决定。发现粗壮果蝇101和147品系成虫之间JHE活性水平的变化过程存在差异，黑腹果蝇Canton S和921283品系成虫之间JHEH活性水平也存在差异。结果表明，101和147品系雌蝇达到一定的JHE活性水平与受精卵产卵的开始高度吻合。文中讨论了JHE在粗壮果蝇繁殖中的可能作用。
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