借助槲皮素和桑色素，采用紫外分光光度法选择性测定亚铁样品中的铁（III）。
Selective determination of Fe(III) in Fe(II) samples by UV-spectrophotometry with the aid of quercetin and morin.
作者信息
Balcerzak Maria, Tyburska Anna, Swiecicka-Füchsel Elzbieta
机构信息
Department of Analytical Chemistry, Warsaw University of Technology, 00-664 Warsaw, Poland.
出版信息
Acta Pharm. 2008 Sep;58(3):327-34. doi: 10.2478/v10007-008-0013-4.
Selective UV-spectrophotometric methods for determination of iron(III) in iron(II) samples have been developed. The methods are based on the interaction of Fe(III) with quercetin and morin, compounds of the flavonoid group. Redox reactions occurring between Fe(III) ions and the reagents used make the basis for the detection. Iron(II) does not react with quercetin and morin under the conditions applied [aqueous-methanolic (3:2) soluions, 0.3 mol L(-1) HCl, 1.2 x 10(-4) mol L(-1) quercetin (morin)] and does not interfere with the determination of Fe(III). Iron(III) can be determined up to 15 microg mL(-1) using both the examined systems. The detection limits are 0.06 and 0.38 microg mL(-1) when using quercetin or morin, respectively. The method with quercetin was applied to the determination of Fe(III) (ca. 0.2%) in a Fe(II) pharmaceutical product.
已开发出用于测定铁（II）样品中铁（III）的选择性紫外分光光度法。这些方法基于铁（III）与黄酮类化合物槲皮素和桑色素的相互作用。铁（III）离子与所用试剂之间发生的氧化还原反应构成了检测的基础。在应用的条件下[水 - 甲醇（3:2）溶液，0.3 mol L⁻¹ HCl，1.2×10⁻⁴ mol L⁻¹ 槲皮素（桑色素）]，铁（II）不与槲皮素和桑色素反应，并且不干扰铁（III）的测定。使用这两种检测体系，铁（III）的测定上限可达15 μg mL⁻¹。分别使用槲皮素或桑色素时，检测限为0.06和0.38 μg mL⁻¹。槲皮素法已应用于测定一种铁（II）药品中铁（III）（约0.2%）的含量。
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