Mardanian S S, Demin Iu M, Nalbandian R M
Biokhimiia. 1977 Jun;42(6):1024-9.
Fluroescent spectra of six b-type ferredoxins of plant and animal origins were obtained. All investigated proteins do not contain tryptophan. The emission maxima of the native proteins, apoproteins prepared by various methods, and denaturated proteins are compared. The effects of pH, ionic strength and ferricyanide on the ferredoxins fluorescence were studied. "Unusual" emission at 340nm noted previously for adrenal ferredoxin was observed for spinach and Chenopodium album ferredoxins too. The localization of tyrosine fluorescent maximum at 340nm in the ferredoxins is not due to interaction of tyrosine with the iron-sulfur center. The data obtained allow to suggest that the tyrosine residues in ferredoxins have different environments.
获得了六种植物和动物来源的b型铁氧化还原蛋白的荧光光谱。所有研究的蛋白质都不含色氨酸。比较了天然蛋白质、通过各种方法制备的脱辅基蛋白和变性蛋白的发射最大值。研究了pH、离子强度和铁氰化物对铁氧化还原蛋白荧光的影响。之前在肾上腺铁氧化还原蛋白中观察到的340nm处的“异常”发射，在菠菜和藜的铁氧化还原蛋白中也被观察到。铁氧化还原蛋白中340nm处酪氨酸荧光最大值的定位并非酪氨酸与铁硫中心相互作用所致。所获得的数据表明，铁氧化还原蛋白中的酪氨酸残基具有不同的环境。
