Cao W W, Wang R F, Slavik M
Department of Animal and Poultry Sciences, University of Arkansas, Fayetteville 72701.
Biotechniques. 1991 May;10(5):574-8.
A simplified dot-blot procedure for screening and subclass isotyping of monoclonal antibodies is described in which only 0.5-50 ng of antigen and 1 microliter of antibody are needed to perform the test. The results on the nitrocellulose membrane can be stored indefinitely for future reference. This method is less expensive, uses smaller quantities of antigen and antibody, and is faster than presently used enzyme-linked immunosorbent assay techniques or other dot-blot methods. Monoclonal antibodies against Mycoplasma gallisepticum were screened and isotyped using this technique.
本文描述了一种用于单克隆抗体筛选和亚类分型的简化斑点印迹程序，该程序仅需0.5-50 ng抗原和1微升抗体即可进行检测。硝酸纤维素膜上的结果可无限期保存以供日后参考。该方法成本较低，使用的抗原和抗体量较少，并且比目前使用的酶联免疫吸附测定技术或其他斑点印迹方法更快。使用该技术筛选并确定了抗鸡毒支原体的单克隆抗体的亚类。
