Morse J W, Boothby J T, Yamamoto R
Avian Dis. 1986 Jan-Mar;30(1):204-6.
A monoclonal antibody against Mycoplasma gallisepticum (MG) (strain S6) was prepared in mice and identified as isotype IgG1 by standard procedures. Although it did react at high titers (1:100,000) in the enzyme-linked immunosorbent assay (the original method for its identification), it failed to react in the agglutination, hemagglutination-inhibition, and growth-inhibition tests. When conjugated to fluorescein isothiocyanate, the monoclonal antibody reacted with the homologous and eight "atypical" strains of MG but not with M. meleagridis or M. synoviae in the direct fluorescent-antibody test. This reagent may be useful for detecting field infections involving atypical strains of MG.
在小鼠体内制备了一种抗鸡毒支原体（MG）（S6株）的单克隆抗体，并通过标准程序鉴定为同型IgG1。尽管它在酶联免疫吸附测定（最初用于鉴定它的方法）中以高滴度（1:100,000）发生反应，但在凝集试验、血凝抑制试验和生长抑制试验中未发生反应。当与异硫氰酸荧光素偶联时，该单克隆抗体在直接荧光抗体试验中与MG的同源菌株和8株“非典型”菌株发生反应，但不与火鸡支原体或滑液支原体发生反应。该试剂可能有助于检测涉及MG非典型菌株的野外感染。
