在雅巴病毒预感染细胞中对猴病毒40 DNA合成的抑制作用。

Inhibition of simian virus 40 DNA synthesis in Yaba virus-preinfected cells.

作者信息

Taylor J L, Rouhandeh H

出版信息

Biochim Biophys Acta. 1977 Mar 18;475(2):276-80. doi: 10.1016/0005-2787(77)90018-1.

PMID:191078

Abstract

The effect of Yaba virus preinfection on DNA synthesis in SV40-infected Jinet cells was studied. Time-course synthesis studies were conducted using the incorporation of labeled thymidine. Yaba virus preinfection resulted in the inhibition of SV40 DNA synthesis when the elapsed time between Yaba virus and SV40 infections was three days. This inhibition was demonstrated by hybridization studies and sedimentation analysis. In addition, the usual stimulation of cellular DNA synthesis induced by SV40 infection was inhibited. This inhibition occurred at a time in Yaba virus infection when no cytoplasmic Yaba virus-specific DNA synthesis occurred.

摘要

研究了雅巴病毒预感染对SV40感染的Jinet细胞中DNA合成的影响。使用标记胸苷掺入法进行了时间进程合成研究。当雅巴病毒和SV40感染之间的间隔时间为三天时，雅巴病毒预感染导致SV40 DNA合成受到抑制。这种抑制通过杂交研究和沉降分析得到证实。此外，SV40感染诱导的细胞DNA合成的正常刺激也受到抑制。这种抑制发生在雅巴病毒感染的某个时间点，此时没有细胞质雅巴病毒特异性DNA合成发生。