宿主细胞核内的雅巴病毒特异性DNA。

Yaba virus-specific DNA in the host cell nucleus.

作者信息

Taylor J L, Rouhandeh H

出版信息

Biochim Biophys Acta. 1977 Sep 6;478(1):59-67. doi: 10.1016/0005-2787(77)90243-x.

PMID:196647

Abstract

DNA-DNA hybridization studies show that Yaba virus-specific DNA is present in the host cell nucleus late in the infection cycle. The nuclear DNA appears to exist as a complete genome, not convalently linked to host cell DNA, as demonstrated by sedimentation analyses. The DNA apperas to be synthesized in the nucleus, since its level of incorporation of label is ten times the background incorporation detectable in the cytoplasm. Extraction of the nuclei by treatment with SDS and EDTA after precipitation with 1 M NaCl separates most of cellular DNA from the Yaba virus-specific DNA.

摘要

DNA-DNA杂交研究表明，在感染周期后期，雅巴病毒特异性DNA存在于宿主细胞核中。沉降分析表明，核DNA似乎以完整基因组的形式存在，并非与宿主细胞DNA共价连接。由于其标记掺入水平是细胞质中可检测到的背景掺入水平的十倍，因此DNA似乎是在细胞核中合成的。在用1M NaCl沉淀后，用SDS和EDTA处理细胞核进行提取，可将大部分细胞DNA与雅巴病毒特异性DNA分离。