Dall'Aglio C, Pascucci L, Mercati F, Giontella A, Pedini V, Scocco P, Ceccarelli P
Dipartimento di Scienze Biopatologiche Veterinarie ed Igiene delle Produzioni Animali ed Alimentari, Sezione di Anatomia Veterinaria, Via San Costanzo 4, 06126, Perugia, Italy.
Eur J Histochem. 2008 Oct-Dec;52(4):229-35. doi: 10.4081/1221.
The presence and distribution of cells positive to orexin A (OXA) and to orexin type 2 receptor (OX2R) were investigated in the gastrointestinal tract of neonatal dogs by means of immunohistochemical techniques. The orexin A-positive cells were identified with some of the endocrine cells in the stomach and in the duodenum; they were both of the open and closed type and were lacking in the large intestine. In the stomach, a large subset of orexin A-positive cells also showed gastrin-like immunoreactivity while, in the duodenum, many of them seemed to store serotonin. The orexin type 2 receptor-positive cells were evidenced all along the gastrointestinal tract examined, also in the large intestine, and they showed the same morphological characteristics as those positive to orexin A. Moreover, the immunohistochemical techniques revealed intense positivity for both orexin A and orexin type 2 receptor in the neurons and fibers of the enteric nervous system. A large subset of orexin A-positive neurons seemed to store substance P.
采用免疫组织化学技术，研究了新生犬胃肠道中食欲素A（OXA）和食欲素2型受体（OX2R）阳性细胞的存在情况和分布。食欲素A阳性细胞在胃和十二指肠中与一些内分泌细胞一致；它们既有开放型也有封闭型，在大肠中不存在。在胃中，大部分食欲素A阳性细胞也显示胃泌素样免疫反应性，而在十二指肠中，许多细胞似乎储存5-羟色胺。在整个被检查的胃肠道中，包括大肠，都证实了食欲素2型受体阳性细胞，它们显示出与食欲素A阳性细胞相同的形态特征。此外，免疫组织化学技术显示，在肠神经系统的神经元和纤维中，食欲素A和食欲素2型受体均呈强阳性。大部分食欲素A阳性神经元似乎储存P物质。
