Sezione di Anatomia Veterinaria, Dipartimento di Scienze Biopatologiche Veterinarie ed Igiene delle Produzioni Animali ed Alimentari, Perugia, Italy.
Acta Histochem. 2012 Feb;114(2):74-8. doi: 10.1016/j.acthis.2011.02.006. Epub 2011 Mar 12.
The aim of the present study was to investigate by immunohistochemistry the presence and distribution of the orexin system in the stomach and gut of fallow deer. Abundant orexin A-positive cells were localized in the middle and basal portions of the mucosal glands of the cardial and fundic regions of the stomach. In the same gastric areas, orexin B-positive cells were also found, mainly localized in the basal portion of glands. In the intestinal tract, orexin-containing cells were occasionally found in the duodenal epithelium and in the rectal intestinal glands. Immunoreactivity for orexin receptors, type 1 and 2 (OX1R and OX2R), was not detected in the same stomach regions. OX1R-immunopositivity was observed in the enteric neuron ganglia localized in the submucosal and muscular intestinal layers, while OX2R-immunopositivity was found close in contact with the cytoplasmic membrane of epithelial cells in the small intestine.
本研究旨在通过免疫组织化学方法研究 fallow deer 胃和肠道中食欲素系统的存在和分布。大量的食欲素 A 阳性细胞定位于胃贲门和胃底部黏膜腺的中、基底部分。在同一胃区，也发现了食欲素 B 阳性细胞，主要定位于腺体的基底部分。在肠道中，十二指肠上皮和直肠肠腺中偶尔发现含有食欲素的细胞。在同一胃区未检测到食欲素受体 1 型和 2 型（OX1R 和 OX2R）的免疫反应性。OX1R 免疫阳性反应见于定位于黏膜和肌层的肠神经元神经节，而 OX2R 免疫阳性反应见于与小肠上皮细胞细胞质膜紧密接触的部位。
