用于葡萄糖生物传感器的纳米多孔氧化铈薄膜
Nanoporous cerium oxide thin film for glucose biosensor.
作者信息
Saha Shibu, Arya Sunil K, Singh S P, Sreenivas K, Malhotra B D, Gupta Vinay
机构信息
Department of Physics & Astrophysics, University of Delhi, Delhi 110007, India.
出版信息
Biosens Bioelectron. 2009 Mar 15;24(7):2040-5. doi: 10.1016/j.bios.2008.10.032. Epub 2008 Nov 17.
Nanoporous cerium oxide (CeO(2)) thin film deposited onto platinum (Pt) coated glass plate using pulsed laser deposition (PLD) has been utilized for immobilization of glucose oxidase (GOx). Atomic force microscopy studies reveal the formation of nanoporous surface morphology of CeO(2) thin film. Response studies carried out using differential pulsed voltammetry (DPV) and optical measurements show that the GOx/CeO(2)/Pt bio-electrode shows linearity in the range of 25-300 mg/dl of glucose concentration. The low value of Michaelis-Menten constant (1.01 mM) indicates enhanced enzyme affinity of GOx to glucose. The observed results show promising application of the nanoporous CeO(2) thin film for glucose sensing application without any surface functionalization or mediator.
采用脉冲激光沉积（PLD）法将纳米多孔氧化铈（CeO₂）薄膜沉积在涂有铂（Pt）的玻璃板上，用于固定葡萄糖氧化酶（GOx）。原子力显微镜研究揭示了CeO₂薄膜纳米多孔表面形态的形成。使用差分脉冲伏安法（DPV）和光学测量进行的响应研究表明，GOx/CeO₂/Pt生物电极在葡萄糖浓度为25 - 300 mg/dl范围内呈线性。米氏常数（1.01 mM）的低值表明GOx对葡萄糖的酶亲和力增强。观察结果表明，纳米多孔CeO₂薄膜在无需任何表面功能化或媒介物的情况下，在葡萄糖传感应用中具有广阔的应用前景。
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