Department of Materials Science and Engineering, Chungnam National University, Daejeon 305-764, Republic of Korea.
Biosens Bioelectron. 2012 Jan 15;31(1):176-81. doi: 10.1016/j.bios.2011.10.013. Epub 2011 Oct 17.
Cerium oxide nanorods (CeO(2) NRs) were synthesized without templates through a low cost and simple non-isothermal precipitation method. The structure and morphology of CeO(2) NRs were characterized by X-ray diffraction and transmission electron microscopy. The CeO(2) NRs films, deposited on indium tin oxide (ITO)-coated glass substrates through electrophoretic deposition, were used for the immobilization of glucose oxidase (GOx). Field emission scanning electron microscopy, Fourier transform infrared spectroscopy, cyclic voltammetry, and electrochemical impedance spectroscopy were used to characterize the CeO(2) NRs/ITO and GOx/CeO(2) NRs/ITO electrodes. The GOx/CeO(2) NRs/ITO electrode exhibits a linear range for the detection of glucose from 2 to 26 mM (correlation coefficient: 0.99) at 1-2s response time. Biosensor sensitivity is 0.165 μA mM(-1) cm(-2) with 100 μM detection limit. The anti-interference ability of the biosensor was also examined. The mediator-less application of CeO(2) NRs for glucose sensing was demonstrated.
氧化铈纳米棒（CeO2 NRs）通过低成本和简单的非等温沉淀法在无模板的情况下合成。CeO2 NRs 的结构和形态通过 X 射线衍射和透射电子显微镜进行了表征。通过电泳沉积将 CeO2 NRs 薄膜沉积在氧化铟锡（ITO）涂覆的玻璃基底上，用于固定葡萄糖氧化酶（GOx）。场发射扫描电子显微镜、傅里叶变换红外光谱、循环伏安法和电化学阻抗谱用于表征 CeO2 NRs/ITO 和 GOx/CeO2 NRs/ITO 电极。GOx/CeO2 NRs/ITO 电极在 1-2s 的响应时间内，对 2-26mM 的葡萄糖检测呈现出线性范围（相关系数：0.99）。生物传感器的灵敏度为 0.165 μA mM-1 cm-2，检测限为 100 μM。还检查了生物传感器的抗干扰能力。证明了 CeO2 NRs 无需介体即可用于葡萄糖传感。
