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一种新型类病毒(CbVd-5)的鉴定与特性分析

Identification and characterization of a new coleviroid (CbVd-5).

作者信息

Hou Wan-Ying, Sano Teruo, Li Feng, Wu Zu-Jian, Li Li, Li Shi-Fang

机构信息

State Key Laboratory of Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Yuanmingyuan West No. 2, Beijing 100193, People's Republic of China.

出版信息

Arch Virol. 2009;154(2):315-20. doi: 10.1007/s00705-008-0276-6. Epub 2008 Dec 27.

Abstract

A viroid-like RNA was detected from coleus (Coleus blumei) in China. It consisted of 274 nucleotides and had 66% sequence identity with a member of the closest known viroid species. The predicted secondary structure is rod-shaped with extensive base pairing, and it has the conserved region characteristic of the genus Coleviroid. Two terminal sequences that are highly conserved among some members of the genus were also identified. The viroid-like RNA was successfully transmitted to coleus by slash-inoculation. This viroid was identified as a new member of the genus Coleviroid, and we tentatively propose the name Coleus blumei viroid 5 (CbVd-5).

摘要

在中国的彩叶草(Coleus blumei)中检测到一种类病毒RNA。它由274个核苷酸组成,与最接近的已知类病毒物种的一个成员具有66%的序列同一性。预测的二级结构呈杆状,具有广泛的碱基配对,并且具有科氏类病毒属的保守区域特征。还鉴定出了该属一些成员中高度保守的两个末端序列。通过划破接种,这种类病毒RNA成功地传播到了彩叶草中。这种类病毒被鉴定为科氏类病毒属的一个新成员,我们暂定其名为彩叶草类病毒5(CbVd-5)。

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