Spieker R L
Max-Planck-Institut für Biochemie, Abteilung Viroidforschung, Martinsried, Federal Republic of Germany.
Arch Virol. 1996;141(11):2153-61. doi: 10.1007/BF01718222.
A viroid was isolated from symptomless Coleus blumei cultivar (cv.) 'Rainbow Gold' plants using the bidirectional PAGE method for analysis of small circular RNA molecules. The viriod was transmitted to viroid-free plants of Coleus blumei cv. 'Scarlet Dragonfly' by mechanical inoculation. Cloning and sequencing revealed that the viriod from the Coleus cv. 'Rainbow Gold' is closely related to the Coleus blumei viriod 1-BvA (CbVd 1-BvA) isolated from the Coleus cv. 'Bienvenue'. Therefore, new viroid sequence variant has been named Coleus blumei viriod 1-RG (CbVd 1-RG). Coleus blumei viriod 1-RG consists of 251 nucleotides, 140 G + C, 111 A + U with a GC content of 55.4%. The most stable rod-like secondary structure of this viroid has 53 G:C, 29 A:U and 5 G:U base pairs with a minimum free energy (at 25 degrees C) of -81.2 kcal/mol (-339.4 kJ/mol). The right terminal domain shows a high sequence similarity to the corresponding domain of hop latent viroid (HLVd).
采用双向聚丙烯酰胺凝胶电泳法分析小环状RNA分子，从无症状的彩叶草品种“彩虹金”植株中分离出一种类病毒。该类病毒通过机械接种传播至无类病毒的彩叶草品种“猩红蜻蜓”植株。克隆和测序结果显示，来自彩叶草品种“彩虹金”的类病毒与从彩叶草品种“欢迎”中分离出的彩叶草类病毒1-BvA（CbVd 1-BvA）密切相关。因此，新的类病毒序列变体被命名为彩叶草类病毒1-RG（CbVd 1-RG）。彩叶草类病毒1-RG由251个核苷酸组成，其中140个为G + C，111个为A + U，GC含量为55.4%。该类病毒最稳定的棒状二级结构有53个G:C、29个A:U和5个G:U碱基对，最小自由能（25摄氏度时）为-81.2千卡/摩尔（-339.4千焦/摩尔）。其右末端结构域与啤酒花潜隐类病毒（HLVd）的相应结构域具有高度序列相似性。
