通过 RT-PCR 检测类病毒。
Detection of Viroids by RT-PCR.
机构信息
Elizabeth Macarthur Agricultural Institute, New South Wales Department of Primary Industries, Menangle, NSW, Australia.
National Citrus Engineering Research Centre, Citrus Research Institute, Southwest University, Chongqing, China.
出版信息
Methods Mol Biol. 2022;2316:143-151. doi: 10.1007/978-1-0716-1464-8_13.
Reverse transcription-polymerase chain reaction (RT-PCR) is an effective method for detecting the presence of viroids in plant tissue. Viroid RNA is converted to cDNA and amplified to detectable levels, making it a fast and useful detection tool, even when the viroid is present at low levels. Methods of viroid detection using conventional RT-PCR are described in this chapter.
逆转录-聚合酶链反应（RT-PCR）是一种有效检测植物组织中类病毒存在的方法。类病毒 RNA 被转化为 cDNA 并扩增到可检测水平，使其成为一种快速有用的检测工具，即使类病毒水平较低时也是如此。本章介绍了使用常规 RT-PCR 进行类病毒检测的方法。
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