Elizabeth Macarthur Agricultural Institute, New South Wales Department of Primary Industries, Menangle, NSW, Australia.
National Citrus Engineering Research Centre, Citrus Research Institute, Southwest University, Chongqing, China.
Methods Mol Biol. 2022;2316:143-151. doi: 10.1007/978-1-0716-1464-8_13.
Reverse transcription-polymerase chain reaction (RT-PCR) is an effective method for detecting the presence of viroids in plant tissue. Viroid RNA is converted to cDNA and amplified to detectable levels, making it a fast and useful detection tool, even when the viroid is present at low levels. Methods of viroid detection using conventional RT-PCR are described in this chapter.
逆转录-聚合酶链反应（RT-PCR）是一种有效检测植物组织中类病毒存在的方法。类病毒 RNA 被转化为 cDNA 并扩增到可检测水平，使其成为一种快速有用的检测工具，即使类病毒水平较低时也是如此。本章介绍了使用常规 RT-PCR 进行类病毒检测的方法。
