Huang Chien-Hsun, Lee Fwu-Ling, Tai Chun-Ju
Bioresource Collection and Research Center, Food Industry Research and Development Institute, 331 Shih-Pin Road, Hsinchu 30062, Taiwan, ROC.
Antonie Van Leeuwenhoek. 2009 Feb;95(2):135-42. doi: 10.1007/s10482-008-9296-1. Epub 2008 Dec 28.
The Saccharomyces sensu stricto complex comprises seven very closely related species. In this study, we compared the use of two different phylogenetic markers, the 26S rDNA and beta-tubulin genes, for discriminating phylogenetic relationships among Saccharomyces sensu stricto strains using sequencing as well as RFLP methods. The average sequence similarity for the beta-tubulin gene (90.0%) among seven strains was significantly less than that for 26S rDNA (98.6%). This result demonstrates that beta-tubulin gene sequences provided higher resolution than 26S rDNA sequences. Species-specific restriction profiles of the Saccharomyces strains were obtained by cutting them with the Tsp509I enzyme. Our data indicate that phylogenetic relationships between these strains are best resolved using sequencing or RFLP analysis of the beta-tubulin gene.
狭义酿酒酵母复合体由七个亲缘关系非常密切的物种组成。在本研究中，我们比较了两种不同的系统发育标记，即26S rDNA和β-微管蛋白基因，采用测序和限制性片段长度多态性（RFLP）方法来鉴别狭义酿酒酵母菌株之间的系统发育关系。七个菌株中β-微管蛋白基因的平均序列相似性（90.0%）显著低于26S rDNA的平均序列相似性（98.6%）。这一结果表明，β-微管蛋白基因序列比26S rDNA序列具有更高的分辨率。通过用Tsp509I酶切割酿酒酵母菌株，获得了物种特异性的限制性图谱。我们的数据表明，使用β-微管蛋白基因的测序或RFLP分析能够最好地解析这些菌株之间的系统发育关系。
