Departamento de Biotecnología, Instituto de Agroquímica y Tecnología de los Alimentos, CSIC, Valencia, Spain.
FEMS Yeast Res. 2010 Jun;10(4):412-21. doi: 10.1111/j.1567-1364.2010.00614.x. Epub 2010 Feb 11.
Saccharomyces kudriavzevii, a yeast species described from a pair of strains isolated from decayed leaves in Japan, has recently been isolated from oak barks in Portugal. Some data suggest that these European S. kudriavzevii populations could be closely related to the S. kudriavzevii genetic background present in natural hybrids isolated from wines and beers in different regions of Europe. However, a more exhaustive study of European S. kudriavzevii natural populations is necessary to confirm this observation. In this study, new S. kudriavzevii isolates were recovered from oak trees in different areas in Spain, and identified and characterized according to their molecular and physiological features. The restriction analysis of 35 nuclear genes amplified by PCR demonstrated that all Spanish S. kudriavzevii isolates exhibit three gene alleles different from those present in the Japanese S. kudriavzevii type strain, but identical to the alleles previously reported for natural S. cerevisiaexS. kudriavzevii hybrids. These results clearly support the hypothesis of a European origin for these natural hybrids.
克鲁维酵母（Saccharomyces kudriavzevii）是一种从日本腐烂叶片中分离出的酵母，最近在葡萄牙的橡木树皮中也被分离出来。一些数据表明，这些欧洲克鲁维酵母种群可能与在欧洲不同地区的葡萄酒和啤酒中分离出的天然杂交种中存在的克鲁维酵母遗传背景密切相关。然而，需要对欧洲克鲁维酵母自然种群进行更详尽的研究，以证实这一观察结果。在这项研究中，从西班牙不同地区的橡木树上回收了新的克鲁维酵母分离株，并根据其分子和生理特征进行了鉴定和特征描述。通过 PCR 扩增的 35 个核基因的限制性分析表明，所有西班牙克鲁维酵母分离株都表现出三种不同于日本克鲁维酵母标准株的基因等位基因，但与先前报道的天然酿酒酵母×克鲁维酵母杂种的等位基因相同。这些结果清楚地支持了这些天然杂种起源于欧洲的假说。
