Ferdman Yael, Sitrit Yaron, Li Yong-Fang, Roth-Bejerano Nurit, Kagan-Zur Varda
Life Sciences Department, Ben Gurion University, Beer-Sheva, Israel.
Antonie Van Leeuwenhoek. 2009 May;95(4):351-62. doi: 10.1007/s10482-009-9321-z. Epub 2009 Mar 12.
Phylogenetic analyses have corroborated the discovery of three internal transcribed spacer (ITS) Types in Terfezia boudieri isolates in the course of earlier studies and have emphasized the divergence of Type 2 from Types 1 and 3. The application of molecular and physiological tools described below, revealed the existence of cryptic species within T. boudieri. The markers used include sequences taken from the 5' end of the ribosomal large subunit gene, a chitin synthase partial sequence, beta-tubulin partial sequence and amplified fragment length polymorphism (AFLP)-based markers. Following initial sequencing of a single PCR amplified sample for each Type, mass analysis of specimens relied on RFLP differences between the Types. Over 100 fruit bodies, 30 or more specimens for each ITS Type, were tested with each of the markers. The markers analysis divided the isolates into three groups, each correlated to a specific ITS Type. Two of the physiological traits examined: mycelial proliferation and mycorrhiza formation, consistently showed responses paralleling the ITS Types; the data presented suggest that T. boudieri is comprised of three cryptic species.
系统发育分析证实了在早期研究过程中在波氏块菌分离物中发现的三种内转录间隔区（ITS）类型，并强调了2型与1型和3型的差异。如下所述的分子和生理工具的应用揭示了波氏块菌中存在隐存种。所使用的标记包括取自核糖体大亚基基因5'端的序列、几丁质合酶部分序列、β-微管蛋白部分序列以及基于扩增片段长度多态性（AFLP）的标记。在对每种类型的单个PCR扩增样品进行初始测序之后，标本的大量分析依赖于各类型之间的限制性片段长度多态性差异。使用每种标记对100多个子实体进行了测试，每种ITS类型测试30个或更多标本。标记分析将分离物分为三组，每组与特定的ITS类型相关。所检测的两个生理特征：菌丝体增殖和菌根形成，始终显示出与ITS类型平行的反应；所呈现的数据表明波氏块菌由三个隐存种组成。
