Bao Leyuan, Redondo Clara, Findlay John B C, Walker John H, Ponnambalam Sreenivasan
Endothelial Cell Biology Unit and Institute of Molecular and Cellular Biology, Leeds Institute of Genetics, Health and Therapeutics, Faculty of Biological Sciences, University of Leeds, Leeds, UK.
Mol Membr Biol. 2009 Apr;26(3):127-35. doi: 10.1080/09687680802637652. Epub 2008 Dec 26.
Membrane protein-protein interactions are important for regulation, targeting, and activity of proteins in membranes but are difficult to detect and analyse. This review covers current approaches to studying membrane protein interactions. In addition to standard biochemical and genetic techniques, the classic yeast nuclear two-hybrid system has been highly successful in identification and characterization of soluble protein-protein interactions. However, classic yeast two-hybrid assays do not work for membrane proteins because such yeast-based interactions must occur in the nucleus. Here, we highlight recent advances in yeast systems for the detection and characterization of eukaryote membrane protein-protein interactions. We discuss these implications for drug screening and discovery.
膜蛋白-蛋白相互作用对于膜中蛋白质的调节、靶向定位及活性而言十分重要，但却难以检测与分析。本综述涵盖了当前研究膜蛋白相互作用的方法。除了标准的生化和遗传学技术外，经典的酵母核双杂交系统在可溶性蛋白-蛋白相互作用的鉴定和表征方面取得了巨大成功。然而，经典的酵母双杂交分析不适用于膜蛋白，因为这类基于酵母的相互作用必须发生在细胞核中。在此，我们重点介绍酵母系统在真核生物膜蛋白-蛋白相互作用检测和表征方面的最新进展。我们还将讨论这些进展在药物筛选与发现中的意义。
