Yadav Seema, Srivastav Ajai Kumar
Department of Zoology, D.D.U. Gorakhpur University, Gorakhpur, India.
Microsc Res Tech. 2009 May;72(5):380-4. doi: 10.1002/jemt.20682.
Pigeons were divided into two numerically equal groups (A and B) containing 30 specimens each. Birds from group A were intraperitoneally injected daily with 0.1 mL/100 g body wt of vehicle (0.1 M acetate buffer, pH 4.3 containing 0.1% gelatin). Specimens from group B were intraperitoneally injected daily with 1 microg/100 g body wt of salmon calcitonin. Six birds were sacrificed from each group 2 h after the last injection on 1st, 3rd, 5th, 10th, and 15th day of the experiment. After collection of blood samples, ultimobranchial and parathyroid glands were fixed for histological studies. In calcitonin-treated C. livia, the plasma calcium levels exhibit a progressive decline from day 1 till day 5. On day 15, the levels become more or less similar to the control value. No change has been noticed on day 1 in the plasma phosphate levels of calcitonin-treated C. livia. The levels decrease progressively from day 3 to day 5; thereafter, it exhibits an elevation so that on day 15, normal plasma phosphate levels is achieved. The ultimobranchial gland of C. livia exhibits no change up to day 5 following calcitonin treatment. The nuclear volume of ultimobranchial cells exhibits a decrease on day 10. This response progresses up to day 15. Few degenerating cells are also discerned following 15 days calcitonin treatment. The parathyroid gland of calcitonin-treated C. livia exhibits no histological alteration up to day 3. The nuclear volume of parathyroidal cells exhibits a progressive increase from day 3 till the close of the experiment (day 15). Moreover, the gland exhibits more compactness on day 10 and day 15. Few degenerating cells are encountered after day 15 following calcitonin treatment.
鸽子被分成两个数量相等的组（A组和B组），每组各有30只样本。A组的鸽子每天腹腔注射0.1 mL/100 g体重的赋形剂（0.1 M醋酸盐缓冲液，pH 4.3，含0.1%明胶）。B组的样本每天腹腔注射1 μg/100 g体重的鲑鱼降钙素。在实验的第1、3、5、10和15天，每组在最后一次注射后2小时处死6只鸽子。采集血样后，将鳃后腺和甲状旁腺固定用于组织学研究。在接受降钙素治疗的家鸽中，血浆钙水平从第1天到第5天呈逐渐下降趋势。在第15天，该水平或多或少与对照值相似。在接受降钙素治疗的家鸽中，第1天血浆磷酸盐水平未发现变化。从第3天到第5天水平逐渐下降；此后，它呈现升高趋势，以至于在第15天达到正常血浆磷酸盐水平。在降钙素治疗后第5天之前，家鸽的鳃后腺没有变化。鳃后腺细胞的核体积在第10天减小。这种反应一直持续到第15天。在降钙素治疗15天后也可观察到少数退变细胞。在接受降钙素治疗的家鸽中，甲状旁腺在第3天之前未出现组织学改变。甲状旁腺细胞的核体积从第3天到实验结束（第15天）呈逐渐增加趋势。此外，该腺体在第10天和第15天表现得更加致密。在降钙素治疗15天后可遇到少数退变细胞。
