Kondo Mika, Yamashita Hiroshi, Uchigashima Mikiko, Kono Takeshi, Takemoto Toshihide, Fujita Masahiro, Saka Machiko, Iwasa Seiji, Ito Shigekazu, Miyake Shiro
Research and Development Department, Medical Electronic Systems Division, Horiba, Miyanohigashi, Kisshoin, Minami-ku, Kyoto, Japan.
J Agric Food Chem. 2009 Jan 28;57(2):359-64. doi: 10.1021/jf802788n.
A direct competitive enzyme-linked immunosorbent assay (dc-ELISA) for the analysis of emamectin residues in agricultural products was developed using a prepared mouse monoclonal antibody. The working range was 0.3-3.0 ng/mL, and the 50% inhibition concentration (IC(50)) was 1.0 ng/mL. The assay was sufficiently sensitive for analysis of the maximum residue limits in agricultural products in Japan (>0.1 microg/g). Emamectin residues contain the following metabolites: the 4''-epi-amino analogue, the 4''-epi-(N-formyl)amino analogue, the 4''-epi-(N-formyl-N-methyl)amino analogue, and the 8,9-Z isomer. The dc-ELISA reacted with these compounds at ratios of 113, 55, 38, and 9.1% of the IC(50) value of emamectin benzoate. Seven kinds of vegetables were spiked with emamectin benzoate at concentrations of 15-300 ng/g, and the recoveries were 91-117% in the dc-ELISA. The dc-ELISA results agreed reasonably well with results obtained by liquid chromatography-tandem mass spectrometry (LC-MS/MS) using spiked samples and actual (incurred) samples. The results indicate that the dc-ELISA was useful for the analysis of emamectin benzoate residues in agricultural products.
利用制备的小鼠单克隆抗体开发了一种用于分析农产品中埃玛菌素残留的直接竞争酶联免疫吸附测定法（dc-ELISA）。其工作范围为0.3 - 3.0 ng/mL，50%抑制浓度（IC(50)）为1.0 ng/mL。该测定法对分析日本农产品中的最大残留限量（>0.1 μg/g）具有足够的灵敏度。埃玛菌素残留包含以下代谢物：4''-表-氨基类似物、4''-表-（N-甲酰基）氨基类似物、4''-表-（N-甲酰基-N-甲基）氨基类似物和8,9-Z异构体。dc-ELISA与这些化合物的反应比例分别为苯甲酸埃玛菌素IC(50)值的113%、55%、38%和9.1%。七种蔬菜中添加了浓度为15 - 300 ng/g的苯甲酸埃玛菌素，在dc-ELISA中的回收率为91 - 117%。dc-ELISA的结果与使用加标样品和实际（受污染）样品通过液相色谱 - 串联质谱法（LC-MS/MS）获得的结果相当吻合。结果表明，dc-ELISA可用于分析农产品中苯甲酸埃玛菌素残留。
