Love L J, Fett J J
Department of Chemistry, Seton Hall University, South Orange, NJ 07079.
J Pharm Biomed Anal. 1991;9(4):323-33. doi: 10.1016/0731-7085(91)80201-j.
Selectivity was optimized for the determination of drugs in urine by direct injection micellar chromatography through changes in specific mobile phase parameters. The rôle of mobile phase pH and the type of surfactant used for mobile phase preparation was investigated. The retention of the urine matrix was found to be minimal between pH 5.5 and 7.5. The non-ionic surfactant, polyoxyethylene 23 lauryl ether (Brij 35), was found to be the surfactant of choice for the separation of drugs from urine. Favourable retention of both the urine background and the analyte was achieved with this surfactant. Micellar mobile phases of optimal composition were used to develop chromatographic procedures for the determination of furosemide, hydrochlorothiazide and propranolol in urine. Good accuracy (98-102% of drug recovered), precision (1-4% RSD) and linearity were obtained for all methods. Limits of detection for all drugs were adequate.
通过改变特定流动相参数，对直接进样胶束色谱法测定尿液中的药物进行了选择性优化。研究了流动相pH值和用于制备流动相的表面活性剂类型的作用。发现在pH 5.5至7.5之间尿液基质的保留最小。发现非离子表面活性剂聚氧乙烯23月桂醚（Brij 35）是从尿液中分离药物的首选表面活性剂。使用这种表面活性剂可实现尿液背景和分析物的良好保留。采用最佳组成的胶束流动相开发了测定尿液中呋塞米、氢氯噻嗪和普萘洛尔的色谱方法。所有方法均具有良好的准确度（药物回收率为98 - 102%）、精密度（相对标准偏差为1 - 4%）和线性。所有药物的检测限都足够。
