Fratczak Agata, Kierzek Ryszard, Kierzek Elzbieta
Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704 Poznan, Poland.
Biochemistry. 2009 Jan 27;48(3):514-6. doi: 10.1021/bi8021069.
Knowledge about the structure of RNA is crucial to understanding its biological activities. Very often, the presence of unusually thermodynamically stable structural fragments in RNAs, such as hairpins, makes it impossible to apply primer extension to visualize the results of chemical mapping experiments. However, replacement of DNA primers with LNA-modified primers overcomes this limitation. This approach was tested successfully on regulatory OxyS RNA and DsrA RNA from Escherichia coli.
了解RNA的结构对于理解其生物学活性至关重要。通常，RNA中存在热力学上异常稳定的结构片段，如发夹结构，使得无法应用引物延伸来可视化化学图谱实验的结果。然而，用LNA修饰的引物替代DNA引物克服了这一限制。这种方法已在大肠杆菌的调控性OxyS RNA和DsrA RNA上成功进行了测试。
