RIKEN Advanced Science Institute, Wako, Saitama 351-0198, Japan.
Bioconjug Chem. 2010 Dec 15;21(12):2276-81. doi: 10.1021/bc1002949. Epub 2010 Nov 19.
Hybridization-sensitive fluorescent DNA probes containing the nucleotide units of locked nucleic acid (LNA) have been developed. Exciton-controlled hybridization-sensitive fluorescent oligonucleotide (ECHO) probes that incorporated LNA nucleotides achieved high thermostability of the hybrid with target RNA strands. The appropriately designed ECHO-LNA chimeric probes exhibited an effective on-off switching property of fluorescence depending on hybridization with RNA and facilitated fluorescent detection of the TAR RNA strand forming a hairpin structure and distinction of one base difference in PLAC4 RNA sequence.
已经开发出了含有锁核酸（LNA）核苷酸单元的杂交敏感荧光 DNA 探针。掺入 LNA 核苷酸的激子控制杂交敏感荧光寡核苷酸（ECHO）探针实现了与靶 RNA 链杂交的高热稳定性。适当设计的 ECHO-LNA 嵌合探针表现出依赖于与 RNA 杂交的有效荧光开-关切换特性，并促进了 TAR RNA 形成发夹结构的荧光检测，以及在 PLAC4 RNA 序列中区分一个碱基差异。
