Hahn P, Taller M, Srubiski L, Kirby L
Department of Obstetrics and Gynaecology, University of British Columbia, Vancouver, Canada.
Biol Neonate. 1991;60(1):1-6. doi: 10.1159/000243382.
We studied the effect of different hormones added in vivo or in vitro on ketogenesis and phosphoenolpyruvate carboxykinase (PEPCK) activity in the small intestinal mucosa of suckling rats. Injection of insulin or dexamethasone in vivo or of an antiglucagon antiserum decreased the rate of ketone formation in the mucosa whereas injection of anti-insulin antiserum led to increased mucosal ketogenesis. PEPCK activity in the mucosa was decreased by the antiglucagon serum but was not affected by insulin or anti-insulin serum injections. Both liver and brown fat PEPCK responded as expected with the activity being elevated by anti-insulin serum and depressed by both insulin and antiglucagon serum. In the in vitro experiments, no effect of any of the agents on PEPCK was found. Ketone formation was suppressed in vitro by insulin or dexamethasone addition to the medium.
我们研究了体内或体外添加不同激素对乳鼠小肠黏膜生酮作用及磷酸烯醇式丙酮酸羧激酶（PEPCK）活性的影响。体内注射胰岛素或地塞米松，或注射抗胰高血糖素抗血清，均可降低黏膜中的酮生成速率，而注射抗胰岛素抗血清则会导致黏膜生酮作用增强。抗胰高血糖素血清可降低黏膜中的PEPCK活性，但胰岛素或抗胰岛素血清注射对其无影响。肝脏和棕色脂肪中的PEPCK活性变化符合预期，抗胰岛素血清可使其活性升高，胰岛素和抗胰高血糖素血清均可使其活性降低。在体外实验中，未发现任何一种试剂对PEPCK有影响。向培养基中添加胰岛素或地塞米松可在体外抑制酮生成。
