Ritland S, Sauar J, Holme R, Blomhoff J P
Clin Chim Acta. 1977 Feb 15;75(1):129-35. doi: 10.1016/0009-8981(77)90508-3.
After incubation whole plasma and low density lipoproteins (LDL) taken before and 10 min after intravenous administration of heparin, from a patient with primary biliary cirrhosis and a patient with familial lecithin:cholesterol acyltransferase (LCAT) deficiency, have been tested for the presence of lipoprotein X (LP-X) by agar gel electrophoresis. LP-X was present in preheparin whole plasma and LDL. No precipitation lines on the cathodal side of the wells, indicating the absence of LP-X, were seen after electrophoresis of postheparin plasma and LDL. Immunodiffusion revealed the presence of apo-beta-lipoproteins and LP-X in preheparin as well as postheparin LDL. After gel filtration three subfractions and similar patterns were observed in the preheparin and postheparin LDL. Electronmicroscopical examination of the intermediate subfractions showed LP-X-like particles in preheparin and postheparin samples. These observations indicate a changed electrophoretic mobility in agar gel of postheparin LP-X, giving a false negative LP-X test by the conventional agar gel electrophoresis.
胆固醇酰基转移酶（LCAT）缺乏症患者，在静脉注射肝素前及注射后10分钟采集的全血血浆和低密度脂蛋白（LDL）进行孵育后，通过琼脂凝胶电泳检测脂蛋白X（LP-X）的存在情况。LP-X存在于肝素注射前的全血血浆和LDL中。肝素注射后血浆和LDL电泳后，在孔的阴极侧未见沉淀线，表明不存在LP-X。免疫扩散显示肝素注射前及注射后的LDL中均存在载脂蛋白β-脂蛋白和LP-X。凝胶过滤后，在肝素注射前和注射后的LDL中观察到三个亚组分及相似的模式。对中间亚组分进行电子显微镜检查，发现在肝素注射前和注射后的样本中均有LP-X样颗粒。这些观察结果表明，肝素注射后LP-X在琼脂凝胶中的电泳迁移率发生了变化，导致传统琼脂凝胶电泳的LP-X检测出现假阴性。
