Chen Zhihang, Song Yanxia, Che Jinjing, Liu Xiaolei, Ning Yu, Shan Chengqi, Hou Yunan, Liu Yunlong, Miao Xiaohong, Cheng Yuanguo
State Key Laboratory of Pathogen and Biosecurity, Beijing, PR China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2009 Feb 1;877(4):408-14. doi: 10.1016/j.jchromb.2008.12.038. Epub 2008 Dec 25.
A sensitive gas chromatographic-mass spectrometric assay was described for determination of beta-elemene and beta-elemenal in human plasma, which has been successfully applied in clinical trial. After liquid-liquid extraction and gas chromatographic separation, the analytes were identified and quantitated. Calibration curves were linear in range from 31.25 to 8000 ng mL(-1) and the limit of quantification for both was 31.25 ng mL(-1). Intra- and inter-day precision at three concentrations were 2.3-8.3% with accuracy of -8.5 to 6.1% for elemene and 3.0-9.9% with accuracy of -2.3 to 5.9% for elemenal. The method was validated to be suitable for further pharmacokinetic study.
描述了一种灵敏的气相色谱 - 质谱分析法，用于测定人血浆中的β-榄香烯和β-榄香烯醇，该方法已成功应用于临床试验。经过液 - 液萃取和气相色谱分离后，对分析物进行鉴定和定量。校准曲线在31.25至8000 ng mL⁻¹范围内呈线性，两者的定量限均为31.25 ng mL⁻¹。三种浓度下的日内和日间精密度，榄香烯为2.3 - 8.3%，准确度为 - 8.5至6.1%；榄香烯醇为3.0 - 9.9%，准确度为 - 2.3至5.9%。该方法经验证适用于进一步的药代动力学研究。
