一种用于同时测定人血浆中β-榄香烯和β-榄香烯醛的灵敏气相色谱-质谱联用方法的验证
Validation of a sensitive gas chromatographic-mass spectrometric method for the simultaneous determination of beta-elemene and beta-elemenal in human plasma.
作者信息
Chen Zhihang, Song Yanxia, Che Jinjing, Liu Xiaolei, Ning Yu, Shan Chengqi, Hou Yunan, Liu Yunlong, Miao Xiaohong, Cheng Yuanguo
机构信息
State Key Laboratory of Pathogen and Biosecurity, Beijing, PR China.
出版信息
J Chromatogr B Analyt Technol Biomed Life Sci. 2009 Feb 1;877(4):408-14. doi: 10.1016/j.jchromb.2008.12.038. Epub 2008 Dec 25.
A sensitive gas chromatographic-mass spectrometric assay was described for determination of beta-elemene and beta-elemenal in human plasma, which has been successfully applied in clinical trial. After liquid-liquid extraction and gas chromatographic separation, the analytes were identified and quantitated. Calibration curves were linear in range from 31.25 to 8000 ng mL(-1) and the limit of quantification for both was 31.25 ng mL(-1). Intra- and inter-day precision at three concentrations were 2.3-8.3% with accuracy of -8.5 to 6.1% for elemene and 3.0-9.9% with accuracy of -2.3 to 5.9% for elemenal. The method was validated to be suitable for further pharmacokinetic study.
描述了一种灵敏的气相色谱 - 质谱分析法，用于测定人血浆中的β-榄香烯和β-榄香烯醇，该方法已成功应用于临床试验。经过液 - 液萃取和气相色谱分离后，对分析物进行鉴定和定量。校准曲线在31.25至8000 ng mL⁻¹范围内呈线性，两者的定量限均为31.25 ng mL⁻¹。三种浓度下的日内和日间精密度，榄香烯为2.3 - 8.3%，准确度为 - 8.5至6.1%；榄香烯醇为3.0 - 9.9%，准确度为 - 2.3至5.9%。该方法经验证适用于进一步的药代动力学研究。
Zotero 插件