用于大鼠肝功能定量评估的三甲双酮耐受性试验。
Trimethadione tolerance test for the quantitative assessment of liver function in rats.
作者信息
Ishikawa A, Fukao K, Tanaka E, Tsuji K, Osada A, Yamamoto Y, Kiyosawa T, Iwasaki Y
机构信息
Department of Surgery, University of Tsukuba, Ibaraki, Japan.
出版信息
J Gastroenterol Hepatol. 1991 Jul-Aug;6(4):363-7. doi: 10.1111/j.1440-1746.1991.tb00872.x.
The quantitative estimation of the hepatic functional volume in rats was attempted using the serum dimethadione (DMO)/trimethadione (TMO) ratio in a single blood sampling after oral administration of TMO, which we call the TMO tolerance test, in order to develop a means of pre-operatively assessing hepatic resectability. Serum DMO/TMO ratios correlated well with the total amount of the hepatic microsomal TMO-N-demethylase activity (enzyme activity) and with the remnant liver weight after 37% and 68% partial hepatectomy. These ratios increased after partial hepatectomy in parallel with the changes in the enzyme activity and the remnant liver weight. The results suggest that the quantitative functional changes of the remnant liver after hepatectomy in both CCl4-treated and untreated rats can be estimated pre-operatively by the TMO tolerance test.
为了开发一种术前评估肝切除可能性的方法，在大鼠口服三甲双酮（TMO）后单次采血，使用血清二甲双酮（DMO）/三甲双酮（TMO）比值尝试对大鼠肝功能体积进行定量评估，我们将此称为TMO耐受试验。血清DMO/TMO比值与肝微粒体TMO - N - 脱甲基酶活性（酶活性）总量以及37%和68%部分肝切除术后的残余肝重量密切相关。部分肝切除术后，这些比值随着酶活性和残余肝重量的变化而平行升高。结果表明，通过TMO耐受试验可以术前估计CCl4处理组和未处理组大鼠肝切除术后残余肝的定量功能变化。
Zotero 插件