Ishikawa A, Tanaka E, Tsuji K, Fukao K, Misawa S, Iwasaki Y
Nihon Geka Gakkai Zasshi. 1987 Mar;88(3):303-7.
Trimethadione (TMO) is exclusively metabolized to dimethadione (DMO) by cytochrome P-450 dependent mono-oxygenase in liver microsomes. We have chosen TMO as an indicator compound for estimating the microsomal function of the liver. TMO tolerance test was performed in the CCl4 intoxicated injury or 68% partial-hepatectomized rats. Serum DMO/TMO ratios in 2 hours following oral administration of TMO in these rats in vivo were well correlated with changes in content of hepatic cytochrome P-450 and activity of TMO N-demethylase in vitro. TMO tolerance test could also determine quantitatively the changes of the functional capacity of the liver in the 68% partial hepatectomy. These results suggest that TMO tolerance test is a useful tool for the surgical indication in the hepatectomy.
三甲双酮（TMO）在肝微粒体中仅通过细胞色素P-450依赖性单加氧酶代谢为二甲双酮（DMO）。我们选择TMO作为评估肝脏微粒体功能的指示化合物。在四氯化碳中毒损伤或68%部分肝切除的大鼠中进行了TMO耐受性试验。在这些大鼠体内口服TMO后2小时的血清DMO/TMO比值与体外肝细胞色素P-450含量和TMO N-脱甲基酶活性的变化密切相关。TMO耐受性试验还可以定量测定68%部分肝切除术中肝脏功能能力的变化。这些结果表明，TMO耐受性试验是肝切除术手术指征的有用工具。
