Chang Shing-Jyh, Huang Tse-Sung, Wang Kung-Liahng, Wang Tao-Yeuan, Yang Yuh-Cheng, Chang Margaret Dah-Tsyr, Wu Yu-Hsuan, Wang Hsei-Wei
Department of Obstetrics and Gynecology, Mackay Memorial Hospital, and National Tsing Hua University, HsinChu, Taiwan.
Taiwan J Obstet Gynecol. 2008 Dec;47(4):422-30. doi: 10.1016/S1028-4559(09)60010-3.
Somatic CD34+ hematopoietic stem/precursor cells (HSPCs) give rise to hematopoietic cells and endothelial cells and have been used in clinical applications. Understanding the genes responsible for stemness and how they interact with each other will help us to manipulate these cells more efficiently in the future.
We performed microarray analysis on human CD34+ HSPCs and on two different progeny cell types, i.e. microvascular endothelial cells and peripheral blood mononuclear cells. Systems biology and advanced bioinformatics tools were used to help clarify the genetic networks associated with these stem cell genes.
We identified CD34+ HSPC genes and found that they were involved in critical biologic processes such as cell cycle regulation, chromosome organization, and DNA repair. We also identified a novel precursor gene cluster on chromosome 19p13.3. Analysis of HSPC-enriched genes using systems biology tools revealed a complex genetic network functioning in CD34+ cells, in which several genes acted as hubs to maintain the stability (such as GATA1) or connectivity (such as hepatic growth factor) of the whole network.
This study provides the foundation for a more detailed understanding of CD34+ HSPCs.
体细胞CD34+造血干/祖细胞（HSPCs）可分化为造血细胞和内皮细胞，并已应用于临床。了解负责干性的基因及其相互作用方式将有助于我们未来更有效地操控这些细胞。
我们对人CD34+ HSPCs以及两种不同的子代细胞类型，即微血管内皮细胞和外周血单个核细胞进行了微阵列分析。运用系统生物学和先进的生物信息学工具来帮助阐明与这些干细胞基因相关的遗传网络。
我们鉴定出了CD34+ HSPC基因，并发现它们参与了诸如细胞周期调控、染色体组织和DNA修复等关键生物学过程。我们还在19号染色体p13.3上鉴定出了一个新的前体基因簇。使用系统生物学工具对富含HSPC的基因进行分析，揭示了一个在CD34+细胞中发挥作用的复杂遗传网络，其中几个基因充当枢纽以维持整个网络的稳定性（如GATA1）或连通性（如肝细胞生长因子）。
本研究为更详细地了解CD34+ HSPCs奠定了基础。
