Ma Ji, Shan Liang, Yu Ling Li, Yuan Chong Gang
Department of Physiology, Life Science Institute, East China Normal University, Shanghai 200062, China.
Fen Zi Xi Bao Sheng Wu Xue Bao. 2008 Oct;41(5):359-66.
The neuropeptid galanin is widely expressed in the central nervous system and has a diverse range of physiological effects including food intaking, arousal/sleep, nociception and reproduction. In this study, expression of galanin and galanin receptors (GalR1 and GalR2) mRNA were identified not only in the neurogenisis regions including subventricular zone (SVZ), rostral migratory stream (RMS) and dentate gyrus (DG) of adult mice but also in the SVZ-derived neural stem cell (NSC) culture. Here, we also showed that the addition of galanin and GalR2-specific agonist Gal2-11 to wild-type or GALKO NSCs under differentiation condition significantly promote the neuritogenesis and increase the length of neurites on the betaIII-tubulin positive cells. This effect could be reduced by treatment of the galanin antagonist M35. These results indicate that galanin and its receptors might regulate neurite extension in differentiating neural stem cells and even participate in the development of the nervous system.
神经肽甘丙肽在中枢神经系统中广泛表达，具有多种生理作用，包括食物摄取、觉醒/睡眠、痛觉感受和生殖。在本研究中，不仅在成年小鼠的神经发生区域，包括脑室下区（SVZ）、吻侧迁移流（RMS）和齿状回（DG）中鉴定出甘丙肽和甘丙肽受体（GalR1和GalR2）mRNA的表达，而且在SVZ衍生的神经干细胞（NSC）培养物中也鉴定出其表达。在此，我们还表明，在分化条件下，向野生型或GALKO神经干细胞中添加甘丙肽和GalR2特异性激动剂Gal2-11可显著促进神经突生成，并增加βIII-微管蛋白阳性细胞上神经突的长度。甘丙肽拮抗剂M35处理可减弱这种作用。这些结果表明，甘丙肽及其受体可能调节分化中的神经干细胞的神经突延伸，甚至参与神经系统的发育。
