Dresang Lindsay R, Vereide David T, Sugden Bill
Department of Oncology, McArdle Laboratory for Cancer Research, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.
J Virol. 2009 Apr;83(7):2930-40. doi: 10.1128/JVI.01974-08. Epub 2009 Jan 7.
We identified binding sites for Epstein-Barr virus (EBV) nuclear antigen 1 (EBNA1) in the human genome using chromatin immunoprecipitation and microarrays. The sequences for these newly identified sites were used to generate a position-weighted matrix (PWM) for EBNA1's DNA-binding sites. This PWM helped identify additional DNA-binding sites for EBNA1 in the genomes of EBV, Kaposi's sarcoma-associated herpesvirus, and cercopithecine herpesvirus 15 (CeHV-15) (also called herpesvirus papio 15). In particular, a homologue of the Rep* locus in EBV was predicted in the genome of CeHV-15, which is notable because Rep* of EBV was not predicted by the previously developed consensus sequence for EBNA1's binding DNA. The Rep* of CeHV-15 functions as an origin of DNA synthesis in the EBV-positive cell line Raji; this finding thus builds on a set of DNA-binding sites for EBNA1 predicted in silico.
我们利用染色质免疫沉淀和微阵列技术在人类基因组中鉴定出了爱泼斯坦-巴尔病毒（EBV）核抗原1（EBNA1）的结合位点。这些新鉴定出的位点序列被用于生成EBNA1 DNA结合位点的位置加权矩阵（PWM）。该PWM有助于在EBV、卡波西肉瘤相关疱疹病毒和猕猴疱疹病毒15（CeHV-15，也称为狒狒疱疹病毒15）的基因组中鉴定出更多EBNA1的DNA结合位点。特别是，在CeHV-15基因组中预测到了EBV中Rep位点的同源物，这一点值得注意，因为EBV的Rep位点并未被先前开发的EBNA1结合DNA的共有序列预测到。CeHV-15的Rep*在EBV阳性细胞系Raji中作为DNA合成的起始点发挥作用；因此，这一发现基于一组通过计算机模拟预测的EBNA1 DNA结合位点。
