硝酸甘油可使猪的冠状动脉舒张，而谷胱甘肽含量或谷胱甘肽S-转移酶活性无变化。
Nitroglycerin relaxes coronary artery of the pig with no change in glutathione content or glutathione S-transferase activity.
作者信息
Sakanashi M, Matsuzaki T, Aniya Y
机构信息
Department of Pharmacology, School of Medicine, Faculty of Medicine, University of the Ryukyus, Okinawa, Japan.
出版信息
Br J Pharmacol. 1991 Aug;103(4):1905-8. doi: 10.1111/j.1476-5381.1991.tb12350.x.
Abstract
- The role of glutathione content and glutathione S-transferase activity in vascular relaxant responses to nitroglycerin was evaluated in potassium (30 mM)-contracted coronary artery strips of the pig by measuring changes in tension, glutathione content and glutathione S-transferase activity. 2. Prior exposure of coronary artery strips to nitroglycerin (10(-5)M or 10(-4)M for 20 min) resulted in tachyphylaxis to subsequent relaxation to nitroglycerin (10(-8)-10(-5)M). 3. The glutathione content and glutathione S-transferase activity of the arterial strips rendered tachyphylactic by prior exposure to nitroglycerin (10(-5)M for 20 min or 10(-3)M for 120 min) were not significantly different from those of control strips. 4. Treatment with diethyl maleate (10(-4)M or 10(-3)M for 60 min) markedly depleted arterial glutathione content in a concentration-dependent manner with no change in glutathione S-transferase activity. 5. The relaxant response of coronary artery strips to nitroglycerin (10(-8)-10(-5)M) was completely unaffected following treatment with diethyl maleate (10(-4)M or 10(-3)M for 60 min). 6. The results suggest that vascular glutathione content does not play an important role in vascular relaxation or tolerance development to nitroglycerin, at least in pig isolated coronary artery.
摘要
- 通过测量张力、谷胱甘肽含量和谷胱甘肽S - 转移酶活性的变化，评估了谷胱甘肽含量和谷胱甘肽S - 转移酶活性在猪的钾（30 mM）收缩冠状动脉条对硝酸甘油血管舒张反应中的作用。2. 冠状动脉条预先暴露于硝酸甘油（10(-5)M或10(-4)M，20分钟）会导致对随后硝酸甘油（10(-8)-10(-5)M）舒张作用产生快速耐受性。3. 预先暴露于硝酸甘油（10(-5)M，20分钟或10(-3)M，120分钟）而产生快速耐受性的动脉条的谷胱甘肽含量和谷胱甘肽S - 转移酶活性与对照条无显著差异。4. 用马来酸二乙酯（10(-4)M或10(-3)M，60分钟）处理以浓度依赖性方式显著降低动脉谷胱甘肽含量，而谷胱甘肽S - 转移酶活性无变化。5. 用马来酸二乙酯（10(-4)M或10(-3)M，60分钟）处理后，冠状动脉条对硝酸甘油（10(-8)-10(-5)M）的舒张反应完全不受影响。6. 结果表明，至少在猪离体冠状动脉中，血管谷胱甘肽含量在血管舒张或对硝酸甘油耐受性形成中不起重要作用。
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