Aoshi Taiki, Carrero Javier A, Konjufca Vjollca, Koide Yukio, Unanue Emil R, Miller Mark J
Department of Pathology and Immunology, Washington University School of Medicine, St Louis, MO 63110, USA.
Eur J Immunol. 2009 Feb;39(2):417-25. doi: 10.1002/eji.200838718.
The spleen is an important organ for the host response to systemic bacterial infections. Many cell types and cell surface receptors have been shown to play role in the capture and control of bacteria, yet these are often studied individually and a coherent picture has yet to emerge of how various phagocytes collaborate to control bacterial infection. We analyzed the cellular distribution of Listeria monocytogenes (LM) in situ during the early phase of infection. Using an immunohistochemistry approach, five distinct phagocyte populations contained LM after i.v. challenge and accounted for roughly all bacterial signal in tissue sections. Our analysis showed that LM was initially captured by a wide range of phagocytes in the marginal zone, where the growth of LM appeared to be controlled. The cellular distribution of LM within phagocyte populations changed rapidly during the first few hours, decreasing in marginal zone macrophages and transiently increasing in CD11c(+) DC. After 4-6 h LM was transported to the periarteriolar lymphoid sheath where the infective foci developed and LM grew exponentially.
脾脏是宿主对全身性细菌感染作出反应的重要器官。许多细胞类型和细胞表面受体已被证明在捕获和控制细菌方面发挥作用，但这些通常是单独研究的，关于各种吞噬细胞如何协作控制细菌感染，尚未形成一个连贯的图景。我们分析了感染早期阶段单核细胞增生李斯特菌（LM）在体内的细胞分布。采用免疫组织化学方法，静脉注射攻击后，五个不同的吞噬细胞群体含有LM，并且几乎占了组织切片中所有的细菌信号。我们的分析表明，LM最初被边缘区的多种吞噬细胞捕获，在那里LM的生长似乎受到控制。在最初几个小时内，LM在吞噬细胞群体中的细胞分布迅速变化，在边缘区巨噬细胞中减少，在CD11c(+)树突状细胞中短暂增加。4-6小时后，LM被转运至动脉周围淋巴鞘，感染灶在那里形成，LM呈指数级生长。
