Baek Kyoung Eun, Yoon Suk Ran, Kim Jong-Tae, Kim Kwang Soo, Kang Seong Ho, Yang Young, Lim Jong-Seok, Choi Inpyo, Nam Myoung Soo, Yoon Michung, Lee Hee Gu
Stem Cell Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea.
Clin Chim Acta. 2009 Mar;401(1-2):128-33. doi: 10.1016/j.cca.2008.12.008. Epub 2008 Dec 14.
Macrophage inhibitory cytokine-1 (MIC-1), a distant member of the transforming growth factor (TGF)-beta superfamily, has been reported to be upregulated and secreted from several cancers. We examined MIC-1 expression and secretion in gastric cancers.
MIC-1 mRNA and protein levels in cancer tissues and cell lines were analyzed by RT-PCR and Western blot. MIC-1 expression in cancer tissues and its secretion in serum were analyzed using immunohistochemistry and ELISA.
MIC-1 was significantly upregulated in gastric cancer tissues and cell lines. MIC-1 was secreted from gastric SNU620 cells and its levels in the serum of cancer patients were 10-fold higher than those of healthy controls. In addition, the staining of MIC-1 expression was strongly increased in metastatic gastric cancers.
MIC-1 was obviously overexpressed in gastric cancers and MIC-1 secretion into blood may be useful for the prediction of gastric cancer progression.
巨噬细胞抑制细胞因子-1（MIC-1）是转化生长因子（TGF）-β超家族的一个远亲成员，据报道在多种癌症中上调并分泌。我们检测了胃癌中MIC-1的表达和分泌情况。
通过逆转录聚合酶链反应（RT-PCR）和蛋白质印迹法分析癌组织和细胞系中MIC-1的信使核糖核酸（mRNA）和蛋白质水平。使用免疫组织化学和酶联免疫吸附测定（ELISA）分析癌组织中MIC-1的表达及其在血清中的分泌情况。
MIC-1在胃癌组织和细胞系中显著上调。MIC-1由胃癌SNU620细胞分泌，癌症患者血清中其水平比健康对照者高10倍。此外，转移性胃癌中MIC-1表达的染色明显增加。
MIC-1在胃癌中明显过表达，MIC-1分泌入血可能有助于预测胃癌进展。
