Lukomski S, Serwecińska L, Rózalski A, Dziadek J, Staczek P, Jaworski A
University of Lódź, Institute of Microbiology, Poland.
Can J Microbiol. 1991 Jun;37(6):419-24. doi: 10.1139/m91-068.
A collection of 45 Proteus penneri strains was characterized with respect to their hemolytic activity and representative cell-free or only cell-bound hemolysin possessing strains were chosen for further study. Extracellular Proteus penneri hemolysin, which was investigated earlier by hybridization, reacted with monospecific antiserum against alpha-hemolysin of Escherichia coli. In this paper we also show, using the colony hybridization technique, that the alpha-hemolysin-like determinant is widely distributed among Proteus penneri strains. Because one of the strains tested, which expressed a high activity of cell-bound hemolytic factor, did not carry such a Hly determinant, the presence of a second hemolysin is postulated. We cannot demonstrate any difference in hybridization patterns of alpha- and beta-hemolytic Proteus penneri strains and accumulation of the toxin molecule inside the cells was also not observed. The existence of another control mechanism, external to the hly operon, for hemolysin gene is suggested.
对45株彭氏变形杆菌菌株进行了溶血活性鉴定，并选择了具有代表性的无细胞或仅细胞结合溶血素的菌株进行进一步研究。先前通过杂交研究的胞外彭氏变形杆菌溶血素与抗大肠杆菌α-溶血素的单特异性抗血清发生反应。在本文中，我们还使用菌落杂交技术表明，α-溶血素样决定簇在彭氏变形杆菌菌株中广泛分布。由于所测试的一株菌株表达了高活性的细胞结合溶血因子，但不携带这种Hly决定簇，因此推测存在第二种溶血素。我们没有发现α-溶血和β-溶血彭氏变形杆菌菌株在杂交模式上有任何差异，也没有观察到毒素分子在细胞内的积累。有人提出，在hly操纵子之外存在另一种溶血素基因的控制机制。
