Finlay B B, Chatfield S, Leung K Y, Dougan G, Falkow S
Biotechnology Laboratory, University of British Columbia, Vancouver, Canada.
Can J Microbiol. 1991 Jul;37(7):568-72. doi: 10.1139/m91-095.
A mutant of Salmonella choleraesuis was identified that could invade (enter) and penetrate through polarized monolayers of Caco-2 and MDCK epithelial cells at normal levels but was defective for intracellular multiplication within these cells. It was also able to survive inside cultured J774 macrophage cells. These bacteria remained inside membrane-bound vacuoles, which coalesced at later times in the perinuclear region of the epithelial cell. This mutant exhibited slightly slower growth rates in rich or minimal media than the parental strain but was normal for iron usage, phosphate usage, and anaerobic growth and was a prototroph. The mutant was completely avirulent when administered orally or intravenously to susceptible mice. These results suggest that the ability to multiply within eukaryotic cells may contribute to S. choleraesuis virulence.
鉴定出一株猪霍乱沙门氏菌突变体，它能够以正常水平侵入并穿透Caco-2和MDCK上皮细胞的极化单层，但在这些细胞内进行细胞内增殖存在缺陷。它也能够在培养的J774巨噬细胞内存活。这些细菌保留在膜结合的液泡内，这些液泡在后期上皮细胞的核周区域聚集。该突变体在丰富或基本培养基中的生长速度比亲本菌株略慢，但在铁利用、磷酸盐利用和厌氧生长方面正常，并且是原养型。当经口或静脉内给予易感小鼠时，该突变体完全无毒力。这些结果表明，在真核细胞内增殖的能力可能有助于猪霍乱沙门氏菌的毒力。
