对斑马鱼中全胚胎原位杂交（WISH）标记的造血细胞群体进行荧光激活细胞分选（FACS）。

Fluorescence-activated cell sorting (FACS) of whole mount in situ hybridization (WISH) labelled haematopoietic cell populations in the zebrafish.

作者信息

Dobson Joseph T, Da'as Sahar, McBride Eileen R, Berman Jason N

机构信息

IWK Health Centre, Dalhousie University, Halifax, NS, Canada.

出版信息

Br J Haematol. 2009 Mar;144(5):732-5. doi: 10.1111/j.1365-2141.2008.07527.x.

DOI:10.1111/j.1365-2141.2008.07527.x

PMID:19133983

Abstract

The zebrafish is a robust animal model for studying vertebrate haematopoiesis and immune cell interactions. However, fluorescence activated cell sorting (FACS) has been limited due to a paucity of available functional zebrafish antibodies. We have developed a technique combining FACS with whole mount in situ hybridization (WISH) that enables the sorting and examining of fixed zebrafish blood cell populations at different stages of embryonic development, providing the opportunity to correlate RNA expression data with cellular morphology.

摘要

斑马鱼是研究脊椎动物造血作用和免疫细胞相互作用的一种强大动物模型。然而，由于可用的功能性斑马鱼抗体数量有限，荧光激活细胞分选（FACS）受到了限制。我们开发了一种将FACS与全胚胎原位杂交（WISH）相结合的技术，该技术能够对胚胎发育不同阶段的固定斑马鱼血细胞群体进行分选和检测，为将RNA表达数据与细胞形态相关联提供了机会。