[CD4+CD25高表达调节性T细胞在妊娠期肝内胆汁淤积症发病机制中的作用]

[Role of CD4+ CD25 high regulatory T cells in the pathogenesis of intrahepatic cholestasis of pregnancy].

作者信息

Lin Li, Zhang Li-Juan

机构信息

Department of Obstetrics and Gynecology, Second Xiangya Hospital, Central South University, Changsha 410011, China.

出版信息

Zhonghua Fu Chan Ke Za Zhi. 2008 Dec;43(12):900-3.

PMID:19134327

Abstract

OBJECTIVE

To investigate the role of CD(4)(+)CD(25)(+) regulatory T cells and CD(4)(+)CD(25)(high) regulatory T cells in peripheral blood and decidua in the pathogenesis of intrahepatic cholestasis of pregnancy (ICP).

METHODS

Peripheral blood and decidua CD(4)(+)CD(25)(+) regulatory T cells and CD(4)(+)CD(25)(high) regulatory T cells in 30 ICP patients (15 mild ICP and 15 severe ICP) and 28 normal pregnant women were analyzed by flow cytometry.

RESULTS

The percentage of CD(4)(+)CD(25)(+) regulatory T cells and CD(4)(+)CD(25)(high) regulatory T cells in the CD(4)(+) T cells population in both peripheral blood and decidua in ICP patients were significantly lower than those in control women [(7.96 +/- 1.32)% vs (17.05 +/- 2.86)%, (17.18 +/- 2.27)% vs (32.01 +/- 3.88)%; (0.78 +/- 0.22)% vs (1.71 +/- 0.69)%, (2.25 +/- 0.89)% vs (8.30 +/- 1.13)%; P < 0.01]. Meanwhile, a significantly higher percentage of CD(4)(+)CD(25)(+) regulatory T cells and CD(4)(+)CD(25)(high) regulatory T cells in the CD(4)(+) T cells population was observed in all decidua samples compared to peripheral blood both in ICP patients (P < 0.01) and in control women (P < 0.01). In addition, the percentages of CD(4)(+)CD(25)(+) regulatory T cells and CD(4)(+)CD(25)(high) regulatory T cells in the CD(4)(+) T cell population in both decidua and peripheral blood in severe ICP [(15.94 +/- 1.95)%, (7.17 +/- 1.17)%, (1.87 +/- 0.90)%, (0.68 +/- 0.19)%] were lower than those in mild ICP [(18.43 +/- 1.90)%, (8.74 +/- 0.96)%, (2.62 +/- 0.72)%, (0.89 +/- 0.20)%] and normal pregnancy (all P < 0.05).

CONCLUSIONS

CD(4)(+)CD(25)(high) regulatory T cell may play an important role in the pathogenesis of ICP and control of disease progression.

摘要

目的

探讨CD4⁺CD25⁺调节性T细胞和CD4⁺CD25⁺高表达调节性T细胞在外周血及蜕膜中在妊娠期肝内胆汁淤积症（ICP）发病机制中的作用。

方法

采用流式细胞术分析30例ICP患者（15例轻度ICP和15例重度ICP）及28例正常孕妇外周血和蜕膜中CD4⁺CD25⁺调节性T细胞和CD4⁺CD25⁺高表达调节性T细胞。

结果

ICP患者外周血和蜕膜中CD4⁺T细胞群体中CD4⁺CD25⁺调节性T细胞和CD4⁺CD25⁺高表达调节性T细胞的百分比均显著低于对照组女性[（7.96±1.32）%对（17.05±2.86）%，（17.18±2.27）%对（32.01±3.88）%；（0.78±0.22）%对（1.71±0.69）%，（2.25±0.89）%对（8.30±1.13）%；P<0.01]。同时，在ICP患者（P<0.01）和对照组女性（P<0.01）中，所有蜕膜样本中CD4⁺T细胞群体中CD4⁺CD25⁺调节性T细胞和CD4⁺CD25⁺高表达调节性T细胞的百分比均显著高于外周血。此外，重度ICP患者蜕膜和外周血中CD4⁺T细胞群体中CD4⁺CD25⁺调节性T细胞和CD4⁺CD25⁺高表达调节性T细胞的百分比[（15.94±1.95）%，（7.17±1.17）%，（1.87±0.90）%，（0.68±0.19）%]低于轻度ICP患者[（18.43±1.90）%，（8.74±0.96）%，（2.62±0.72）%，（0.89±0.20）%]和正常妊娠组（均P<0.05）。