Beauchamp R D, Coffey R J, Lyons R M, Perkett E A, Townsend C M, Moses H L
Department of Surgery, University of Texas Medical Branch, Galveston 77550.
Cancer Res. 1991 Oct 1;51(19):5253-60.
A serotonin-secreting human pancreatic carcinoid cell line (BON) is demonstrated to express transcripts for all three mammalian types of transforming growth factor beta (TGF beta 1, 2, and 3). Similarly, freshly excised carcinoid tumors from six patients were also found to express mRNA for all three of the type-beta TGFs. Medium conditioned by BON cells had detectable TGF beta activity, although most of the activity was latent as determined by radioreceptor assay with and without prior acid treatment. However, nonactivated BON-conditioned medium stimulated DNA synthesis, soft agar growth, and an increase in TGF beta 1 and fibronectin mRNA expression in AKR-2B fibroblasts. In addition, BON-conditioned medium had a potent endothelial cell growth-stimulatory activity. Since the TGF beta s inhibit growth of endothelial cells, the presence of other growth factors was suspected. TGF alpha, c-sis, and basic fibroblast growth factor transcripts were also found to be expressed by the BON carcinoid cells. These data indicate that multiple peptide growth factors may have a paracrine role in the desmoplastic reaction accompanying carcinoid tumors.
一种分泌血清素的人胰腺类癌细胞系（BON）被证明可表达三种哺乳动物类型的转化生长因子β（TGFβ1、2和3）的转录本。同样，在来自六名患者的新鲜切除的类癌肿瘤中也发现可表达所有三种β型TGF的mRNA。由BON细胞条件培养的培养基具有可检测到的TGFβ活性，尽管通过有无预先酸处理的放射受体测定法确定，大部分活性是潜伏的。然而，未激活的BON条件培养基刺激了AKR-2B成纤维细胞中的DNA合成、软琼脂生长以及TGFβ1和纤连蛋白mRNA表达的增加。此外，BON条件培养基具有强大的内皮细胞生长刺激活性。由于TGFβ会抑制内皮细胞的生长，因此怀疑存在其他生长因子。还发现BON类癌细胞表达TGFα、c-sis和碱性成纤维细胞生长因子转录本。这些数据表明，多种肽生长因子可能在类癌肿瘤伴随的促结缔组织增生反应中起旁分泌作用。
