Kashama Johnny, Prince Véronique, Simao-Beaunoir Anne-Marie, Beaulieu Carole
Centre d'Etude et de Valorisation de la Diversité Microbienne, Département de Biologie, Université de Sherbrooke, Sherbrooke, QC, J1K 2R1, Canada.
J Ind Microbiol Biotechnol. 2009 Mar;36(3):391-9. doi: 10.1007/s10295-008-0509-4. Epub 2009 Jan 10.
Forty-one bacterial strains isolated from the headbox water of two machines in a Canadian paper mill were associated with the genera Asticcacaulis, Acidovorax, Bacillus, Exiguobacterium, Hydrogenophaga, Pseudomonas, Pseudoxanthomonas, Staphylococcus, Stenotrophomonas based on the sequence of their 16S rRNA genes. The metabolic profile of these strains were determined using Biolog EcoPlate, and the bacteria were divided into four metabolic groups. Metabolic profiles of the bacterial communities colonizing the headbox water of two paper machines was also determined weekly over a 1 year period. The only compound that was not reduced by the bacterial community was 2-hydroxybenzoic acid. Utilization frequency of the other carbon sources in the Biolog EcoPlate ranged from 3 to 100%. The metabolic profiles of the bacterial community did not vary considerably between the two paper machines. However, the metabolic profile varied among the sampling dates.
根据16S rRNA基因序列，从加拿大一家造纸厂两台机器的流浆箱水中分离出的41株细菌菌株与鞘氨醇单胞菌属、食酸菌属、芽孢杆菌属、嗜冷栖热菌属、嗜氢菌属、假单胞菌属、假黄单胞菌属、葡萄球菌属、嗜麦芽窄食单胞菌属有关。使用Biolog EcoPlate测定了这些菌株的代谢谱，这些细菌被分为四个代谢组。在一年的时间里，还每周测定一次在两台造纸机流浆箱水中定殖的细菌群落的代谢谱。细菌群落唯一未还原的化合物是2-羟基苯甲酸。Biolog EcoPlate中其他碳源的利用频率在3%至100%之间。两台造纸机之间细菌群落的代谢谱没有显著差异。然而，代谢谱在不同采样日期之间有所不同。
