Rubin O, Crettaz D, Canellini G, Tissot J-D, Lion N
Service Régional Vaudois de Transfusion Sanguine, Lausanne, Switzerland.
Vox Sang. 2008 Nov;95(4):288-97. doi: 10.1111/j.1423-0410.2008.01101.x.
Microparticles (MPs) are small phospholipid vesicles of less than 1 microm, shed in blood flow by various cell types. These MPs are involved in several biological processes and diseases. MPs have also been detected in blood products; however, their role in transfused patients is unknown. The purpose of this study was to characterize those MPs in blood bank conditions.
Qualitative and quantitative experiments using flow cytometry or proteomic techniques were performed on MPs derived from erythrocytes concentrates. In order to count MPs, they were either isolated by various centrifugation procedures or counted directly in erythrocyte concentrates.
A 20-fold increase after 50 days of storage at 4 degrees C was observed (from 3370 +/- 1180 MPs/microl at day 5 to 64 850 +/- 37 800 MPs/microl at day 50). Proteomic analysis revealed changes of protein expression comparing MPs to erythrocyte membranes. Finally, the expression of Rh blood group antigens was shown on MPs generated during erythrocyte storage.
Our work provides evidence that storage of red blood cell is associated with the generation of MPs characterized by particular proteomic profiles. These results contribute to fundamental knowledge of transfused blood products.
微粒（MPs）是直径小于1微米的小磷脂囊泡，由多种细胞类型在血流中释放。这些微粒参与多种生物学过程和疾病。在血液制品中也检测到了微粒；然而，它们在输血患者中的作用尚不清楚。本研究的目的是在血库条件下对这些微粒进行表征。
对来自红细胞浓缩物的微粒进行了使用流式细胞术或蛋白质组学技术的定性和定量实验。为了计数微粒，它们要么通过各种离心程序分离，要么直接在红细胞浓缩物中计数。
在4℃储存50天后观察到增加了20倍（从第5天的3370±1180个微粒/微升增加到第50天的64850±37800个微粒/微升）。蛋白质组学分析揭示了与红细胞膜相比微粒蛋白质表达的变化。最后，在红细胞储存过程中产生的微粒上显示了Rh血型抗原的表达。
我们的工作提供了证据，表明红细胞的储存与具有特定蛋白质组特征的微粒的产生有关。这些结果有助于对输血血液制品的基础知识的了解。
