Roy S G, Bhisey A N
Cancer Research Institute, Tata Memorial Centre, Bombay, India.
Cell Biol Int Rep. 1991 May;15(5):377-87. doi: 10.1016/0309-1651(91)90126-4.
Regulation of cytoplasmic microtubule complex (CMTC) organization was studied in cultured human fibroblasts and mouse macrophages by somatic cell fusion. The heterokaryons stained with antitubulin antibody had fibroblast-like CMTC even 72 hours after fusion. There was no change in CMTC pattern when more than one macrophage had fused with one fibroblast. However, the macrophage CMTC was expressed in heterokaryons when the former were located at the periphery of the heterokaryon. To evaluate the role of existing CMTC in determining the CMTC of heterokaryons, the heterokaryons were treated with nocodazole to depolymerize the CMTC and then allowed to recover. The resultant CMTC was fibroblast like.
通过体细胞融合，在培养的人成纤维细胞和小鼠巨噬细胞中研究了细胞质微管复合体（CMTC）组织的调控。用抗微管蛋白抗体染色的异核体即使在融合后72小时仍具有成纤维细胞样的CMTC。当一个以上的巨噬细胞与一个成纤维细胞融合时，CMTC模式没有变化。然而，当巨噬细胞位于异核体的周边时，其CMTC会在异核体中表达。为了评估现有CMTC在决定异核体CMTC中的作用，用诺考达唑处理异核体以使CMTC解聚，然后使其恢复。产生的CMTC呈成纤维细胞样。
