Ozden Hilmi, Tekin Neslihan, Akyuz Fahrettin, Gurer Firdevs, Ustuner Mehmet C, Kucuk Fulya, Guven Gul, Yaylak Faik
Department of Anatomy, Faculty of Medicine, Eskisehir Osmangazi University, Eskisehir, Turkey.
Saudi Med J. 2009 Jan;30(1):30-4.
To determine on protective role of NG-nitro-L-arginine methyl ester L-NAME, and insulin on the liver in streptozotocin STZ induced diabetic rats.
This study was performed in the Department of Biochemistry, Faculty of Medicine, Eskisehir Osmangazi University, Eskisehir, Turkey in 2007. Forty male Wistar albino rats were divided into 5 groups. These were untreated, diabetic control, STZ+insulin, STZ+L-NAME and STZ+insulin+L-NAME induced groups. The STZ was intraperitonally injected into 3 groups, and includes insulin, L-NAME, and their joint administrations as protective agents. The blood glucose and nitric oxide (NO) levels were determined. The tissue samples were obtained at the end of the fourth week. The liver tissue distortions were evaluated using hematoxylin and eosin staining.
The serum glucose level was significantly higher in diabetic control (p=0.000), than the untreated group. Nitric oxide level was significantly lower in STZ+L-NAME (p=0.000) than the untreated group. The focal pseudo lobular structures without vena centralis increased portal fibrillary necrosis, and bile duct stenosis with coagulation necrosis of the peripheral hepatocytes were more observed in diabetic group than the protective agent groups. In addition, insulin, and L-NAME lead to hepatocyte regeneration; and minimal mononuclear cell infiltration was noted.
NG-nitro-L-arginine methyl ester inhibits NO level in STZ+L-NAME induced group. NG-nitro-L-arginine methyl ester either alone, or with insulin combination significantly attenuates the liver morphological disarrangements in STZ induced diabetic rats.
确定NG-硝基-L-精氨酸甲酯（L-NAME）和胰岛素对链脲佐菌素（STZ）诱导的糖尿病大鼠肝脏的保护作用。
本研究于2007年在土耳其埃斯基谢希尔奥斯曼加齐大学医学院生物化学系进行。40只雄性Wistar白化大鼠分为5组。分别为未治疗组、糖尿病对照组、STZ+胰岛素组、STZ+L-NAME组和STZ+胰岛素+L-NAME诱导组。对3组大鼠腹腔注射STZ，并将胰岛素、L-NAME及其联合给药作为保护剂。测定血糖和一氧化氮（NO）水平。在第四周结束时获取组织样本。使用苏木精和伊红染色评估肝组织病变。
糖尿病对照组的血清葡萄糖水平显著高于未治疗组（p=0.000）。STZ+L-NAME组的一氧化氮水平显著低于未治疗组（p=0.000）。糖尿病组比保护剂组更易观察到无中央静脉的局灶性假小叶结构增加、门静脉纤维性坏死以及外周肝细胞凝固性坏死伴胆管狭窄。此外，胰岛素和L-NAME可导致肝细胞再生；且可见少量单核细胞浸润。
NG-硝基-L-精氨酸甲酯可抑制STZ+L-NAME诱导组中的NO水平。NG-硝基-L-精氨酸甲酯单独使用或与胰岛素联合使用均可显著减轻STZ诱导的糖尿病大鼠肝脏的形态紊乱。
